ROI Thresholding Methods in Endogenous fluorophore images.

Example Crop from 100X confocal images of endogenous synaptic marker in the dorsal nerve cord (Neurexin::Skylan-S) with annotated ROIs (green) from different methods showing: i. The raw crop with a region highlighted; ii. A zoom in of the raw crop in th...

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Bibliographic Details
Main Author:	Araven Tiroumalechetty (17827252) (author)
Other Authors:	Elisa B. Frankel (1866058) (author), Peri T. Kurshan (17827258) (author)
Published:	2025
Subjects:	Biochemistry Cell Biology Biological Sciences not elsewhere classified Information Systems not elsewhere classified image analysis workflow image analysis pipelines puncta often suffer 2d fluorescent puncta div >< p automated unbiased detection fluorescent puncta unbiased detection often hampered accurate analysis widespread adoption visualization options synapses residing specific samples restriction parameters provides robust originally developed nerve cords ideal metric fluorescence imaging existing methods enabling users elegans </ data acquisition critical tasks correlation metrics code approach characterize images channel images
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ROI Thresholding Methods in Endogenous fluorophore images.

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