Ethylene alters the metabolic balance and stimulates poly-hydroxybutyrate (PHB) accumulation.
<p><b>A)</b> Cells were grown with no nitrogen source in the presence and absence of 100 ppb ethylene for 24 hours at which time samples were processed and real-time RT-qPCR used to measure the transcript levels of the indicated genes. Data were normalized to expression of housekee...
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2025
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| Summary: | <p><b>A)</b> Cells were grown with no nitrogen source in the presence and absence of 100 ppb ethylene for 24 hours at which time samples were processed and real-time RT-qPCR used to measure the transcript levels of the indicated genes. Data were normalized to expression of housekeeping genes and then to expression in the absence of ethylene as described in the materials and methods. Data is the average ± SEM. * <i>p</i> value < 0.05; ** <i>p</i> value < 0.01 compared to the no ethylene control as determined by Student’s t-test. <b>B)</b> Cells were grown overnight with 10 mM of the indicated nitrogen source or no added nitrogen with no shaking. At this time RNA was extracted and real-time RT-qPCR used to measure the transcript level of <i>Azoetr1</i>. Data was normalized to levels in the presence of NaNO<sub>3</sub> and housekeeping genes as described in the materials and methods. Data is the average ± SEM. Statistical difference from NaNO<sub>3</sub> was determined with ANOVA. n.s. not significant; * <i>p</i> value < 0.05; ** <i>p</i> value < 0.01. <b>C, D)</b> Cells were grown in MMAB in the presence and absence of 100 ppb ethylene for 24 hours at which time the cells were stained with Nile red. C) Representative DIC merged with fluorescent images of cells in control cells treated with 100 ppb ethylene. Scale bar = 1 µM. D) Stack graph showing the number of PHB granules accumulated per cell in control (n = 389) and ethylene-treated cells (n = 251).</p> |
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