Determination of cilia length upon Wnt/β-catenin signaling activation.
<p><b>(A)</b> Quantification of ciliary length of RPE1 cells treated with serum-free medium (control), Co-CM and Wnt3a-CM and serum starved for 16 h. The box/dot plots show quantification of ciliary length from three independent experiments. Control, <i>n</i> = 90; Co-C...
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2025
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| Summary: | <p><b>(A)</b> Quantification of ciliary length of RPE1 cells treated with serum-free medium (control), Co-CM and Wnt3a-CM and serum starved for 16 h. The box/dot plots show quantification of ciliary length from three independent experiments. Control, <i>n</i> = 90; Co-CM, <i>n</i> = 95; Wnt3a-CM, <i>n</i> = 47. <b>(B)</b> Quantification of ciliary length of RPE1 cells treated with buffer control (<i>n</i> = 95) or purified surrogate Wnt agonist (<i>n</i> = 175) after 16 h of serum starvation. The box/dot plots show quantification of ciliary length from three independent experiments. <b>(C)</b> Quantification of <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003369#pbio.3003369.g002" target="_blank">Fig 2B</a> showing the relative signal intensity of p-DVL2 to actin in siLUC and siTCF7 Wnt3a-CM treated samples. The bar graph indicates the mean ± S.D. of three independent experiments. <i>P</i> values are based on Student <i>t</i> test. The data underlying the graphs in this figure can be found in the S2 Data file.</p> <p>(S3_Fig.TIF)</p> |
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