Generation of COWP6-mScarlet-I and COWP7-mScarlet-I strains.

A) Strategy to target the C-terminus of COWP6 (cgd4_3090) for fusion with the mScarlet-I-3 × myc Repair Cassette (mScarlet-I in magenta and 3 × myc in blue). NanoLuciferase-Neomycin resistance fusion protein (NLuc-NeoR) expressed by the constitutive...

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Bibliographic Details
Main Author:	Ross Bacchetti (16752855) (author)
Other Authors:	Sarah Stevens (398830) (author), Leandro Lemgruber (2882552) (author), Mariana Azevedo Gonzalez Oliva (22486492) (author), Emma M. Sands (16752861) (author), Konstantinos Alexandrou (22486495) (author), Michele Tinti (75217) (author), Lee Robinson (9651716) (author), Jack C. Hanna (16752846) (author), Simona Seizova (16752852) (author), Peyton Goddard (22486498) (author), Massimo Vassalli (2178036) (author), Mattie Christine Pawlowic (22486501) (author)
Published:	2025
Subjects:	Biochemistry Microbiology Cell Biology Genetics Ecology Immunology Infectious Diseases Computational Biology Space Science Environmental Sciences not elsewhere classified Physical Sciences not elsewhere classified diarrhoeal disease contributing biomechanical measurements determine 2 – 9 common water treatments typical oocyst morphology oocyst wall formation hardy oocyst wall produce viable oocysts div >< p oocyst wall proteins oocyst wall effective treatments oocyst suture young children work confirms using crispr true components technical challenges substantial morbidity significant cause secrete material resists chlorination remaining members puncta consistent organelles store often waterborne microscopy confirms knockout oocysts including chlorination fluorescent fusions cryptosporidium </ cowp8 </ cowp1 </ cowp </
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Generation of COWP6-mScarlet-I and COWP7-mScarlet-I strains.

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