Mod antibody and RNAi validations.
<p><b>(A)</b> Merged image depicting Mod protein localization using anti-Mod antibody (blue), together with the nucleolar marker Fibrillarin (Fib; green) and NB marker Miranda (Mir; red), in a Control NB (1407><i>yw</i>). Individual channels are depicted in greyscale...
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2024
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| Summary: | <p><b>(A)</b> Merged image depicting Mod protein localization using anti-Mod antibody (blue), together with the nucleolar marker Fibrillarin (Fib; green) and NB marker Miranda (Mir; red), in a Control NB (1407><i>yw</i>). Individual channels are depicted in greyscale in <b>(B-D)</b>. <b>(E)</b> Merged image of staining of <i>modL8</i> NBs using the same antibodies and imaging settings as above with individual channels represented in <b>(F-H)</b>. NBs in <i>modL8</i> brains are negative for Mod signal and total signal is abrogated. Scale bars represent 5μm. <b>(I-P)</b> Larval brains immunostained with Mir (Red), Fib (Green), and Mod (Blue) in Control <b>(I-L)</b> or <i>modRNAi</i> <b>(M-P)</b> using identical antibodies and imaging settings. RNAi expression decreases Mod signal in NBs. Scale bars represent 10μm.</p> <p>(TIFF)</p> |
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