Supplementary file 1_Mixed nitrogen forms enhance growth and photosynthetic nitrogen use efficiency by optimizing nitrogen metabolism and leaf N allocation in Gisela 6 cherry rootstock.docx
<p>Nitrogen (N) is the most important nutrient for plant growth and development. However, the mechanisms by which the form and supply of N regulate the growth and N utilization of cherry rootstock are unclear at present. We investigated the effects of different N supply levels and N forms on t...
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2025
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| _version_ | 1849927635994411008 |
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| author | Xinxiang Xu (8354664) |
| author2 | Yan Tang (195081) Liu He (246513) Yanxia Sun (4346239) Daliang Liu (391967) Yuxia Wang (2383) Fangdong Li (6555272) Lingling Zhao (488465) Laiqing Song (5771750) Fen Wang (285388) Yanju Li (9697993) Xu Zhang (49484) |
| author2_role | author author author author author author author author author author author |
| author_facet | Xinxiang Xu (8354664) Yan Tang (195081) Liu He (246513) Yanxia Sun (4346239) Daliang Liu (391967) Yuxia Wang (2383) Fangdong Li (6555272) Lingling Zhao (488465) Laiqing Song (5771750) Fen Wang (285388) Yanju Li (9697993) Xu Zhang (49484) |
| author_role | author |
| dc.creator.none.fl_str_mv | Xinxiang Xu (8354664) Yan Tang (195081) Liu He (246513) Yanxia Sun (4346239) Daliang Liu (391967) Yuxia Wang (2383) Fangdong Li (6555272) Lingling Zhao (488465) Laiqing Song (5771750) Fen Wang (285388) Yanju Li (9697993) Xu Zhang (49484) |
| dc.date.none.fl_str_mv | 2025-11-25T06:24:46Z |
| dc.identifier.none.fl_str_mv | 10.3389/fpls.2025.1696713.s001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Supplementary_file_1_Mixed_nitrogen_forms_enhance_growth_and_photosynthetic_nitrogen_use_efficiency_by_optimizing_nitrogen_metabolism_and_leaf_N_allocation_in_Gisela_6_cherry_rootstock_docx/30704036 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Plant Biology nitrate ammonium nitrogen metabolism sweet cherry rootstock photosynthetic nitrogen use efficiency |
| dc.title.none.fl_str_mv | Supplementary file 1_Mixed nitrogen forms enhance growth and photosynthetic nitrogen use efficiency by optimizing nitrogen metabolism and leaf N allocation in Gisela 6 cherry rootstock.docx |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <p>Nitrogen (N) is the most important nutrient for plant growth and development. However, the mechanisms by which the form and supply of N regulate the growth and N utilization of cherry rootstock are unclear at present. We investigated the effects of different N supply levels and N forms on the growth, N uptake, assimilation and distribution, and photosynthetic N use efficiency (PNUE) of Gisela 6 cherry rootstock seedlings. The results showed that a high N level and a single supply of either nitrate N or ammonium N hindered N uptake and assimilation, increased photosynthetic limitation, reduced PNUE and <sup>15</sup>N use efficiency, and inhibited cherry rootstock growth. Further experiments showed that a mixed supply of nitrate N and ammonium N maintained high transcription levels of nitrate and ammonium transporters as well as N metabolism enzyme activities, thereby increasing the net inflow rates of NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> into roots and the soluble protein content of leaves. In addition, a mixed N supply reduced oxidative damage to leaves by maintaining an appropriate nitrate/ammonium ratio, increased the proportion of leaf N allocated to photosynthetic N, decreased leaf cell wall thickness, and enhanced stomatal conductance, mesophyll conductance, and the maximum carboxylation efficiency. This resulted in reduced leaf photosynthetic limitation, increased leaf net photosynthetic rate and PNUE, and ultimately enhanced the growth of Gisela 6 cherry rootstock seedlings. Our results provide a basis for optimizing N management strategies in cherry cultivation.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_bf4a2a2c6a65a6a64cd698f929ba6a43 |
| identifier_str_mv | 10.3389/fpls.2025.1696713.s001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30704036 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Supplementary file 1_Mixed nitrogen forms enhance growth and photosynthetic nitrogen use efficiency by optimizing nitrogen metabolism and leaf N allocation in Gisela 6 cherry rootstock.docxXinxiang Xu (8354664)Yan Tang (195081)Liu He (246513)Yanxia Sun (4346239)Daliang Liu (391967)Yuxia Wang (2383)Fangdong Li (6555272)Lingling Zhao (488465)Laiqing Song (5771750)Fen Wang (285388)Yanju Li (9697993)Xu Zhang (49484)Plant Biologynitrateammoniumnitrogen metabolismsweet cherry rootstockphotosynthetic nitrogen use efficiency<p>Nitrogen (N) is the most important nutrient for plant growth and development. However, the mechanisms by which the form and supply of N regulate the growth and N utilization of cherry rootstock are unclear at present. We investigated the effects of different N supply levels and N forms on the growth, N uptake, assimilation and distribution, and photosynthetic N use efficiency (PNUE) of Gisela 6 cherry rootstock seedlings. The results showed that a high N level and a single supply of either nitrate N or ammonium N hindered N uptake and assimilation, increased photosynthetic limitation, reduced PNUE and <sup>15</sup>N use efficiency, and inhibited cherry rootstock growth. Further experiments showed that a mixed supply of nitrate N and ammonium N maintained high transcription levels of nitrate and ammonium transporters as well as N metabolism enzyme activities, thereby increasing the net inflow rates of NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> into roots and the soluble protein content of leaves. In addition, a mixed N supply reduced oxidative damage to leaves by maintaining an appropriate nitrate/ammonium ratio, increased the proportion of leaf N allocated to photosynthetic N, decreased leaf cell wall thickness, and enhanced stomatal conductance, mesophyll conductance, and the maximum carboxylation efficiency. This resulted in reduced leaf photosynthetic limitation, increased leaf net photosynthetic rate and PNUE, and ultimately enhanced the growth of Gisela 6 cherry rootstock seedlings. Our results provide a basis for optimizing N management strategies in cherry cultivation.</p>2025-11-25T06:24:46ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fpls.2025.1696713.s001https://figshare.com/articles/dataset/Supplementary_file_1_Mixed_nitrogen_forms_enhance_growth_and_photosynthetic_nitrogen_use_efficiency_by_optimizing_nitrogen_metabolism_and_leaf_N_allocation_in_Gisela_6_cherry_rootstock_docx/30704036CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307040362025-11-25T06:24:46Z |
| spellingShingle | Supplementary file 1_Mixed nitrogen forms enhance growth and photosynthetic nitrogen use efficiency by optimizing nitrogen metabolism and leaf N allocation in Gisela 6 cherry rootstock.docx Xinxiang Xu (8354664) Plant Biology nitrate ammonium nitrogen metabolism sweet cherry rootstock photosynthetic nitrogen use efficiency |
| status_str | publishedVersion |
| title | Supplementary file 1_Mixed nitrogen forms enhance growth and photosynthetic nitrogen use efficiency by optimizing nitrogen metabolism and leaf N allocation in Gisela 6 cherry rootstock.docx |
| title_full | Supplementary file 1_Mixed nitrogen forms enhance growth and photosynthetic nitrogen use efficiency by optimizing nitrogen metabolism and leaf N allocation in Gisela 6 cherry rootstock.docx |
| title_fullStr | Supplementary file 1_Mixed nitrogen forms enhance growth and photosynthetic nitrogen use efficiency by optimizing nitrogen metabolism and leaf N allocation in Gisela 6 cherry rootstock.docx |
| title_full_unstemmed | Supplementary file 1_Mixed nitrogen forms enhance growth and photosynthetic nitrogen use efficiency by optimizing nitrogen metabolism and leaf N allocation in Gisela 6 cherry rootstock.docx |
| title_short | Supplementary file 1_Mixed nitrogen forms enhance growth and photosynthetic nitrogen use efficiency by optimizing nitrogen metabolism and leaf N allocation in Gisela 6 cherry rootstock.docx |
| title_sort | Supplementary file 1_Mixed nitrogen forms enhance growth and photosynthetic nitrogen use efficiency by optimizing nitrogen metabolism and leaf N allocation in Gisela 6 cherry rootstock.docx |
| topic | Plant Biology nitrate ammonium nitrogen metabolism sweet cherry rootstock photosynthetic nitrogen use efficiency |