lncCyt b cooperates with hnRNPA2B1 to modulate glycolysis and exhibits physiological significance.
<p>(A) Native RIP followed by RT-qPCR detecting MDL1AS, lncND5 and lncCyt b retrieved by anti-hnRNPA2B1 antibody, or by normal IgG and anti-P53 antibody, in whole-cell, nuclear, or cytoplasmic lysates of A549 cells. The retrieved RNAs are presented as a percentage of the amount input. Data are...
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2025
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| Summary: | <p>(A) Native RIP followed by RT-qPCR detecting MDL1AS, lncND5 and lncCyt b retrieved by anti-hnRNPA2B1 antibody, or by normal IgG and anti-P53 antibody, in whole-cell, nuclear, or cytoplasmic lysates of A549 cells. The retrieved RNAs are presented as a percentage of the amount input. Data are shown as means ± SD of n = 3 independent experiments. (B) Venn diagram showing overlap of nuclear genes regulated by hnRNPA2B1 and lncCyt b in A549 cells. (C) GO enrichment analysis of the nuclear genes co-regulated by hnRNPA2B1 and lncCyt b. (D) Glycolytic stress test showing the reduced glycolytic capacity of A549 cells by hnRNPA2B1 or lncCyt b knockdown. Data are shown as means ± SD of n = 3 independent experiments. *<i>P</i> < 0.05 by Student’s <i>t</i> test. (E) CCK-8 assays showing the decreased proliferation of A549 cells by lncCyt b or hnRNPA2B1 knockdown. Data are shown as means ± SD of n = 3 independent experiments. ***<i>P</i> < 0.001 by Student’s <i>t</i> test. (F) Transwell assays showing the weakened migration and invasion of A549 cells by lncCyt b or hnRNPA2B1 knockdown. Scale bars, 100 μm.</p> |
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