Removal of IKK in microglia induces changes in microglia number and morphology during prion infection.

Removal of IKK in microglia induces changes in microglia number and morphology during prion infection.

<p>Terminal mice with IKK KO microglia were compared to wpi-matched WT infected mice. <b>A</b> Brains were stained for Iba1 + microglia, which were counted and compared in the <b>B</b> cortex, <b>C</b> hippocampus, <b>D</b> thalamus and <b>...

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Manylion Llyfryddiaeth
Prif Awdur:	Arielle J. D. Hay (15456341) (author)
Awduron Eraill:	Katriana A. Popichak (12093647) (author), Genova Mumford (15456344) (author), Jifeng Bian (354060) (author), Payton Shirley (21568259) (author), Lauren Wolfrath (21568262) (author), Michael Eggers (21568265) (author), Eric M. Nicholson (8262054) (author), Ronald B. Tjalkens (3281031) (author), Mark D. Zabel (15456359) (author), Julie A. Moreno (7647266) (author)
Cyhoeddwyd:	2025
Pynciau:	Medicine Microbiology Cell Biology Genetics Molecular Biology Neuroscience Physiology Immunology Cancer Infectious Diseases iκb kinase complex cellular stress signals irreversible neuronal loss associated behavioral deficits sc </ sup prion disease pathogenesis prion disease early div >< p cellular prion protein inflammatory signaling molecules infected brain homogenates terminal wt mice induced glial inflammation accelerated disease terminal animals protein homeostasis inflammatory cytokines induced transcription accelerated loss prion diseases signaling pathway intracranially infected infected wild infected brain b signaling specific pathology significantly lower significantly increased significantly downregulated poorly understood nuclear factor near ablation microglial survival key regulator kappa b infectious form increased density including pro host protection hippocampal neurons glial cells forms aggregates findings present cell survival associated genes
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Crynodeb:	<p>Terminal mice with IKK KO microglia were compared to wpi-matched WT infected mice. <b>A</b> Brains were stained for Iba1 + microglia, which were counted and compared in the <b>B</b> cortex, <b>C</b> hippocampus, <b>D</b> thalamus and <b>E</b> cerebellum for 9 animals with IKK KO microglia, 6 wpi-matched WT and 3 terminal WT animals. Scale bars = 20·m. <b>F</b> Representative images of Iba1 + hippocampal microglia morphological skeletons between groups. Scale bar = 10·m. <b>G</b> Microglia cartoon depicting features analyzed via skeletonization, created with Biorender.com. <b>H</b> Process length, <b>I</b> number of process branches and <b>J</b> number of process endpoints was compared between hippocampal microglia from WT mice and those with IKK KO microglia. Skeletonization and C3 analysis were performed on 3 randomly selected animals per group. Welch’s t-test, error bars = SEM, <i>p</i> < 0.01, **<i>p </i>< 0.0001.</p>