Figure 5 from Exploiting Natural Killer Cell Engagers to Control Pediatric B-cell Precursor Acute Lymphoblastic Leukemia
<p>NKCEs override HLA-I inhibitory interactions. Degranulation activity of NK cells (All NK, gating on CD3<sup>−</sup>CD56<sup>+</sup>) and different NK-cell subsets [single (s)KIR2DL1<sup>+</sup>, KIR2DL3<sup>+</sup>/KIR3DL1<sup>+</sup&...
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2025
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| Riassunto: | <p>NKCEs override HLA-I inhibitory interactions. Degranulation activity of NK cells (All NK, gating on CD3<sup>−</sup>CD56<sup>+</sup>) and different NK-cell subsets [single (s)KIR2DL1<sup>+</sup>, KIR2DL3<sup>+</sup>/KIR3DL1<sup>+</sup>, and KIR<sup>−</sup>NKG2A<sup>+</sup>, identified by appropriate gating strategy reported in Supplementary Fig. S1] from healthy donors (<i>n</i> = 3) or transplanted patient (<i>n</i> = 1) upon 4-hour coculture with MHH-CALL-4 cells in presence of IC-NKp46- or CD19-NKp46-NKCE at 10<sup>0</sup> μg/mL. Results from three independent experiments are reported. <b>A,</b> Contour plot of a representative donor showing the degranulation of different NK-cell subsets in the presence of the target cells and NKCEs. Numbers indicate the percentage of cells in each quadrant. <b>B,</b> Data from 3 healthy donors are reported. <b>C</b> and <b>D,</b> Degranulation of NK-cell subsets from 1 patient 1-year after haplo-HSCT. <b>C,</b> Contour plot of flow cytometry (raw data). <b>D,</b> Graphical representation.</p> |
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