HUWE1 enhances WNT signaling through a mechanism independent of changes in CTNNB1 abundance.
<p>The same cell lines were used in A-F. Each circle represents a unique clonal cell line (determined by genotyping, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011677#pgen.1011677.s009" target="_blank">S1 File</a>). A single valu...
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2025
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| Summary: | <p>The same cell lines were used in A-F. Each circle represents a unique clonal cell line (determined by genotyping, <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011677#pgen.1011677.s009" target="_blank">S1 File</a>). A single value for the parental WT HAP1-7TGP cell line, and the average value from 3 independent clonal cell lines for each of the other genotypes, all relative to untreated WT HAP1-7TGP cells, are indicated by a horizontal line and quantified above each group of circles. WT HAP1-7TGP cells were treated with 50% WNT3A CM for 24 hr where indicated. Significance was determined by unpaired t-test with Welch’s correction. (A) Relative soluble CTNNB1 abundance (CTNNB1 intensity normalized to total protein and GAPDH intensity, average from duplicate immunoblots shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1011677#pgen.1011677.s002" target="_blank">S2B Fig</a>) in MFS of the indicated cell lines. (B) Relative WNT reporter activity (median EGFP fluorescence from 100,000 singlets). (C-F) Relative WNT target gene expression (average quantification of <i>AXIN2</i>, <i>RNF43</i>, <i>TNFRSF19</i>, or <i>NKD1</i> mRNA normalized to <i>HPRT1</i> mRNA, each measured in triplicate reactions).</p> |
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