Immunoblotting confirmation that β-actin-PafA-Myc ligates 3 × Flag-TbN-PupE substrate to HA-AMOT.

(A) Validation of the labeling efficiency of TbC-PupE and TbN-PupE on interacting proteins by PafA. (B) Immunoblotting confirming that actin-PafA-Myc ligates the 3 × Flag-TbN-PupE substrate onto exogenous AMOT-HA. 3 × Flag-TbN-PupE stably expressed cell...

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Hlavní autor:	Lin Xie (136792) (author)
Další autoři:	Lijuan Gao (5093777) (author), Weihong Fu (8430690) (author), Gangyun Wu (22676687) (author), Hua Li (46469) (author), Wenxiu Ning (18239607) (author)
Vydáno:	2025
Témata:	Biophysics Biochemistry Cell Biology Genetics Molecular Biology Physiology Biotechnology Developmental Biology Cancer Inorganic Chemistry Infectious Diseases Plant Biology Virology Chemical Sciences not elsewhere classified step enzymatic reaction offering new insights capturing intermediary proteins capture intermediary proteins biotinylate intermediary proteins plit -< u div >< p labeling intermediary proteins tu </ u proximity labeling tools proximity labeling tool characterized protein triads bmp signaling pathways p </ u bridge two non </ u signaling pathways interacting proteins tools available powerful tool two non protein interactions key components interacting partners identifying upstream downstream effectors disease pathogenesis directly label
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Immunoblotting confirmation that β-actin-PafA-Myc ligates 3 × Flag-TbN-PupE substrate to HA-AMOT.

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