GPX4 inhibition abolished the protective effects of PMS on HG
<p> + PA-induced Min6 cell damage. (a) Cell viability was detected with a MTT assay, RSL-3 abolished the protective effects of PMS on HG + PA-induced Min6 cell injury. (b-e) RSL-3 abolished the effects of PMS on ROS (b), MDA (c), 4-HNE (d), and total Iron (e) in HG + PA -induced Min6 cells. (f...
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2025
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| 總結: | <p> + PA-induced Min6 cell damage. (a) Cell viability was detected with a MTT assay, RSL-3 abolished the protective effects of PMS on HG + PA-induced Min6 cell injury. (b-e) RSL-3 abolished the effects of PMS on ROS (b), MDA (c), 4-HNE (d), and total Iron (e) in HG + PA -induced Min6 cells. (f-i) RT-qPCR analysis showed that RSL-3 abolished the effects of PMS on mRNA expression of <i>Acsl4</i> (f), <i>Trf</i> (g), <i>Steap3</i> (h) and <i>Ftl1</i> (i). (j-n) Western blot results showed that RSL-3 abolished the effects of PMS on protein levels of ACSL4 (j, k), TRF (j, l) STEAP3 (j, m), and FTL (j, n). Data are presented as the mean ± SD, n = 6 for a-e; n = 3 for f-n. ##: <i>p</i> < 0.01 and #: <i>p</i> < 0.05 as compared to the NC group; **: <i>p</i> < 0.01, and *: <i>p</i> < 0.05, as compared to the HG + PA group; ns: no significant (<i>p </i>> 0.05).</p> |
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