GPX4 inhibition abolished the protective effects of PMS on HG

<p> + PA-induced Min6 cell damage. (a) Cell viability was detected with a MTT assay, RSL-3 abolished the protective effects of PMS on HG + PA-induced Min6 cell injury. (b-e) RSL-3 abolished the effects of PMS on ROS (b), MDA (c), 4-HNE (d), and total Iron (e) in HG + PA -induced Min6 cells. (f...

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Hlavní autor:	Hongmin Zhao (20710544) (author)
Další autoři:	Renlin Li (21439962) (author), Xuan Guo (1791202) (author), Jingrui Kang (21578315) (author), Huajun Li (8309904) (author), Xiaoyun Wang (148866) (author), Yuansong Wang (14103603) (author), Huantian Cui (5798996) (author), Shuquan Lv (11897978) (author), Weibo Wen (8115296) (author), Zhongyong Zhang (11887698) (author)
Vydáno:	2025
Témata:	Biophysics Biochemistry Cell Biology Genetics Molecular Biology Pharmacology Biotechnology Immunology Cancer Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified mechanisms remain unclear pancreatic &# 946 upregulated factors linked t2dm ), may improving t2dm outcomes pancreatic islet β alleviating cellular damage ferroptosis inhibitor ferrostatin div >< p gpx4 inhibitor rsl ferroptosis inhibitor fer pms alleviated hg t2dm via activation gpx4 pathway modulation pancreatic β factors associated t2dm mice gpx4 pathway gpx4 axis cells via tissue damage induced damage induce damage diminish damage cell damage suppressing ferroptosis suppressed ferroptosis reduced ferroptosis inhibiting ferroptosis inhibit ferroptosis vivo </ vitro </ shows promise positive control pms exhibited palmitic acid lipid peroxidation key pathology glutamate transporter findings revealed beneficial impacts 3 attenuated
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Shrnutí:	<p> + PA-induced Min6 cell damage. (a) Cell viability was detected with a MTT assay, RSL-3 abolished the protective effects of PMS on HG + PA-induced Min6 cell injury. (b-e) RSL-3 abolished the effects of PMS on ROS (b), MDA (c), 4-HNE (d), and total Iron (e) in HG + PA -induced Min6 cells. (f-i) RT-qPCR analysis showed that RSL-3 abolished the effects of PMS on mRNA expression of <i>Acsl4</i> (f), <i>Trf</i> (g), <i>Steap3</i> (h) and <i>Ftl1</i> (i). (j-n) Western blot results showed that RSL-3 abolished the effects of PMS on protein levels of ACSL4 (j, k), TRF (j, l) STEAP3 (j, m), and FTL (j, n). Data are presented as the mean ± SD, n = 6 for a-e; n = 3 for f-n. ##: <i>p</i> < 0.01 and #: <i>p</i> < 0.05 as compared to the NC group; *: <i>p</i> < 0.01, and : <i>p</i> < 0.05, as compared to the HG + PA group; ns: no significant (<i>p </i>> 0.05).</p>

GPX4 inhibition abolished the protective effects of PMS on HG

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