Growth curve of <i>M. smegmatis</i> PLJR962-eccD3-gRNA strain with linezolid and rifampicin.
<p>Growth curves of <i>M. smegmatis mc</i><sup><i>2</i></sup> 155 wild type strain (diamonds, inner graph a and b), <i>M. smegmatis</i> PLJR962-<i>eccD3</i>-gRNA strain (circles) (a) and (b) without (gray line) and with (black line) A...
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2025
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| Summary: | <p>Growth curves of <i>M. smegmatis mc</i><sup><i>2</i></sup> 155 wild type strain (diamonds, inner graph a and b), <i>M. smegmatis</i> PLJR962-<i>eccD3</i>-gRNA strain (circles) (a) and (b) without (gray line) and with (black line) ATc 0.0002 μM (100 ng/mL), and <i>M. smegmatis</i> PLJR962-control-gRNA strain (triangles) (c and d) without (gray line) and with (black line) ATc 0.0002 μM (100 ng/mL), and with a final concentration of linezolid of 0.001 µM (0.5 μg/mL) (a), and with a final concentration of rifampicin of 0.03 μM (0.8 μg/mL) (b), and (20 μg/mL) of kanamycin. A significant difference was observed in the linezolid and rifampicin concentrations in <i>M. smegmatis</i> PLJR962-<i>eccD3</i>-gRNA strain without or with ATc from 20 to 27 hours. An unpaired two-tailed Student’s <i>t</i>-test was used and <i>p</i> < 0.01 (**) were obtained (<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1012936#pcbi.1012936.s020" target="_blank">S8 Table</a> and MDdb Science Gateway at <a href="http://md-db.org" target="_blank">http://md-db.org</a> with entry ID 690005 <i>in-vitro</i> data). <i>M. smegmatis</i> mc<sup>2</sup> 155 wild type growth curve were included in the inner graph. Each experiment was performed in technical triplicates showing the average data with the standard deviation.</p> |
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