GC-MS analyses for LadA homologs.

GC-MS analyses for LadA homologs.

<p>Assayed reaction conditions are as follows: (A) <i>Wild-type</i> N-Strep-tag® LadA homolog from <i>Pseudomonas</i> sp. ANT H4 (WP_149412683.1) (18 μM), <i>wild-type</i> N-Strep-tag® Fre (6 μM), hexadecane (1 mM, t<sub>R</sub> = 12.74 min), FMN...

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Bibliografiske detaljer
Hovedforfatter:	Raul Mireles (21175708) (author)
Andre forfattere:	Arne Matthews (22279026) (author), Robin Teufel (1355409) (author), Lianet Noda-García (22279029) (author)
Udgivet:	2025
Fag:	Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Biotechnology Cancer Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified two distinct metal functionalization step could four novel homologs environmentally friendly processes despite extensive efforts >- alkane hydroxylation >- alkane functionalization significant challenge due >- alkane monooxygenases enzyme families known div >< p scalable industrial applications >- alkanes flavoprotein monooxygenases findings challenge environmental applications vitro </ soluble proteins similar conclusions protein superfamily optimized variant one iron n </ inherent stability free flavin first oxo dependent enzymes current understanding covalent bonds binding protein
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