Pseudo-time differentiation trajectory analysis of <i>Trem2</i><sup><i>+</i></sup><i>Fcrls</i><sup><i>+</i></sup> and <i>Spp1</i><sup><i>+</i></sup><i>Cav1</i><sup><i>+</i></sup> macrophages from MRL and B6.
<p>(A) Pseudo-time trajectory analysis was conducted to determine potential origin of <i>Trem2</i><sup><i>+</i></sup><i>Fcrls</i><sup><i>+</i></sup> <i>and Spp1</i><sup><i>+</i></sup><...
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2025
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| Summary: | <p>(A) Pseudo-time trajectory analysis was conducted to determine potential origin of <i>Trem2</i><sup><i>+</i></sup><i>Fcrls</i><sup><i>+</i></sup> <i>and Spp1</i><sup><i>+</i></sup><i>Cav1</i><sup><i>+</i></sup> macrophages from <i>Ly6c2</i><sup><i>+</i></sup> monocytes or tissue resident macrophages. The relative position of cells across the pseudo-time differentiation trajectory is depicted in the figure. Each point is a cell and is colored according to its cluster identity. For both MRL and B6, cells along the trajectory were divided into six groupings based on experimental timepoints (D0-4W). An expansion of <i>Ly6c2</i><sup><i>+</i></sup> monocytes along the trajectory towards macrophages was observed after injury, primarily at D1 and D3 in both strains (indicated by arrows). B) Superimposition of the expression of monocyte marker <i>Ly6c2</i> on the pseudo-time trajectory. Each point is a cell and is colored according to its pseudo-time value. Circle size represents the gene expression level. C) Superimposition of the expression of <i>Arg1</i>, a gene specifically enriched in <i>Spp1</i><sup><i>+</i></sup><i>Cav1</i><sup><i>+</i></sup> macrophages at D1-D3, on the pseudo-time trajectory. Expansion of cell populations expressing high levels of <i>Arg1</i> in the monocyte to macrophage direction was observed after injury (indicated by arrows).</p> |
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