Auto-diploidization of haploid <i>Candida tropicalis</i> cells.
<p>(<b>A</b>) Colony and cellular morphologies of the haploid and auto-diploid strains. Fungal cells were plated onto YPD medium and incubated at 30 °C for 4 days. Scale bars: 1 mm for colonies; 5 μm for cells. (<b>B</b>) FACS analysis of the genomic DNA content. (<b...
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2025
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| Summary: | <p>(<b>A</b>) Colony and cellular morphologies of the haploid and auto-diploid strains. Fungal cells were plated onto YPD medium and incubated at 30 °C for 4 days. Scale bars: 1 mm for colonies; 5 μm for cells. (<b>B</b>) FACS analysis of the genomic DNA content. (<b>C</b>) PCR verification of the <i>MTL</i> locus. The <i>MTL</i><b>a</b>/α diploid (JX1016) strain served as the control. (<b>D</b>) Frequency of auto-diploidization. Haploid strains were plated onto YPD medium and incubated at 30 °C for 4 days. Then, all colonies were collected for DNA content analyses using flow cytometry assays. Frequency of auto-diploidization = total number of diploid colonies/total number of colonies × 100%. Three biological replicates were performed. (<b>E</b>) Growth curves. Approximately 1 × 10<sup>6</sup> cells from a single colony were inoculated into liquid YPD medium and incubated at 30 °C. The growth state was monitored by measuring the OD<sub>600</sub> at the indicated time points. Three biological replicates of each strain were performed. The data underlying this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3003062#pbio.3003062.s012" target="_blank">S3 Data</a>. The flow cytometry files are available from the Figshare database (<a href="https://doi.org/10.6084/m9.figshare.28350296" target="_blank">https://doi.org/10.6084/m9.figshare.28350296</a>).</p> |
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