Table1_Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.XLSX
<p>Our knowledge of the assembly and dynamics of the cytokinetic contractile ring (CR) in animal cells remains incomplete. We have previously used super-resolution light microscopy and platinum replica electron microscopy to elucidate the ultrastructural organization of the CR in first divisio...
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| مؤلفون آخرون: | , , , , , , , , |
| منشور في: |
2024
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| _version_ | 1852026333698195456 |
|---|---|
| author | John H. Henson (9649061) |
| author2 | Gabriela Reyes (433340) Nina T. Lo (19749666) Karina Herrera (19749669) Quenelle W. McKim (19749672) Hannah Y. Herzon (19749675) Maritriny Galvez-Ceron (19749678) Alexandra E. Hershey (19749681) Rachael S. Kim (19749684) Charles B. Shuster (1912285) |
| author2_role | author author author author author author author author author |
| author_facet | John H. Henson (9649061) Gabriela Reyes (433340) Nina T. Lo (19749666) Karina Herrera (19749669) Quenelle W. McKim (19749672) Hannah Y. Herzon (19749675) Maritriny Galvez-Ceron (19749678) Alexandra E. Hershey (19749681) Rachael S. Kim (19749684) Charles B. Shuster (1912285) |
| author_role | author |
| dc.creator.none.fl_str_mv | John H. Henson (9649061) Gabriela Reyes (433340) Nina T. Lo (19749666) Karina Herrera (19749669) Quenelle W. McKim (19749672) Hannah Y. Herzon (19749675) Maritriny Galvez-Ceron (19749678) Alexandra E. Hershey (19749681) Rachael S. Kim (19749684) Charles B. Shuster (1912285) |
| dc.date.none.fl_str_mv | 2024-09-27T08:29:59Z |
| dc.identifier.none.fl_str_mv | 10.3389/fcell.2024.1483345.s002 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Table1_Cytokinetic_contractile_ring_structural_progression_in_an_early_embryo_positioning_of_scaffolding_proteins_recruitment_of_-actinin_and_effects_of_myosin_II_inhibition_XLSX/27117495 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Cell Biology Marine Biology Cell Development, Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) cytokinesis contractile ring myosin II septin anillin actin sea urchin α-actinin |
| dc.title.none.fl_str_mv | Table1_Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.XLSX |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <p>Our knowledge of the assembly and dynamics of the cytokinetic contractile ring (CR) in animal cells remains incomplete. We have previously used super-resolution light microscopy and platinum replica electron microscopy to elucidate the ultrastructural organization of the CR in first division sea urchin embryos. To date, our studies indicate that the CR initiates as an equatorial band of clusters containing myosin II, actin, septin and anillin, which then congress over time into patches which coalesce into a linear array characteristic of mature CRs. In the present study, we applied super-resolution interferometric photoactivated localization microscopy to confirm the existence of septin filament-like structures in the developing CR, demonstrate the close associations between septin2, anillin, and myosin II in the CR, as well as to show that septin2 appears consistently submembranous, whereas anillin is more widely distributed in the early CR. We also provide evidence that the major actin cross-linking protein α-actinin only associates with the linearized, late-stage CR and not with the early CR clusters, providing further support to the idea that α-actinin associates with actomyosin structures under tension and can serve as a counterbalance. In addition, we show that inhibition of actomyosin contraction does not stop the assembly of the early CR clusters but does arrest the progression of these structures to the aligned arrays required for functional cytokinesis. Taken together our results reinforce and extend our model for a cluster to patch to linear structural progression of the CR in sea urchin embryos and highlight the evolutionary relationships with cytokinesis in fission yeast.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_cf6eff81a5d0d7d0ade99bc080893e5e |
| identifier_str_mv | 10.3389/fcell.2024.1483345.s002 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/27117495 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Table1_Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.XLSXJohn H. Henson (9649061)Gabriela Reyes (433340)Nina T. Lo (19749666)Karina Herrera (19749669)Quenelle W. McKim (19749672)Hannah Y. Herzon (19749675)Maritriny Galvez-Ceron (19749678)Alexandra E. Hershey (19749681)Rachael S. Kim (19749684)Charles B. Shuster (1912285)Cell BiologyMarine BiologyCell Development, Proliferation and DeathCell MetabolismCell NeurochemistryCellular Interactions (incl. Adhesion, Matrix, Cell Wall)cytokinesiscontractile ringmyosin IIseptinanillinactinsea urchinα-actinin<p>Our knowledge of the assembly and dynamics of the cytokinetic contractile ring (CR) in animal cells remains incomplete. We have previously used super-resolution light microscopy and platinum replica electron microscopy to elucidate the ultrastructural organization of the CR in first division sea urchin embryos. To date, our studies indicate that the CR initiates as an equatorial band of clusters containing myosin II, actin, septin and anillin, which then congress over time into patches which coalesce into a linear array characteristic of mature CRs. In the present study, we applied super-resolution interferometric photoactivated localization microscopy to confirm the existence of septin filament-like structures in the developing CR, demonstrate the close associations between septin2, anillin, and myosin II in the CR, as well as to show that septin2 appears consistently submembranous, whereas anillin is more widely distributed in the early CR. We also provide evidence that the major actin cross-linking protein α-actinin only associates with the linearized, late-stage CR and not with the early CR clusters, providing further support to the idea that α-actinin associates with actomyosin structures under tension and can serve as a counterbalance. In addition, we show that inhibition of actomyosin contraction does not stop the assembly of the early CR clusters but does arrest the progression of these structures to the aligned arrays required for functional cytokinesis. Taken together our results reinforce and extend our model for a cluster to patch to linear structural progression of the CR in sea urchin embryos and highlight the evolutionary relationships with cytokinesis in fission yeast.</p>2024-09-27T08:29:59ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fcell.2024.1483345.s002https://figshare.com/articles/dataset/Table1_Cytokinetic_contractile_ring_structural_progression_in_an_early_embryo_positioning_of_scaffolding_proteins_recruitment_of_-actinin_and_effects_of_myosin_II_inhibition_XLSX/27117495CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/271174952024-09-27T08:29:59Z |
| spellingShingle | Table1_Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.XLSX John H. Henson (9649061) Cell Biology Marine Biology Cell Development, Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) cytokinesis contractile ring myosin II septin anillin actin sea urchin α-actinin |
| status_str | publishedVersion |
| title | Table1_Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.XLSX |
| title_full | Table1_Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.XLSX |
| title_fullStr | Table1_Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.XLSX |
| title_full_unstemmed | Table1_Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.XLSX |
| title_short | Table1_Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.XLSX |
| title_sort | Table1_Cytokinetic contractile ring structural progression in an early embryo: positioning of scaffolding proteins, recruitment of α-actinin, and effects of myosin II inhibition.XLSX |
| topic | Cell Biology Marine Biology Cell Development, Proliferation and Death Cell Metabolism Cell Neurochemistry Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) cytokinesis contractile ring myosin II septin anillin actin sea urchin α-actinin |