DataSheet1_Assessing sepsis-induced immunosuppression to predict positive blood cultures.pdf
Introduction<p>Bacteremia is a life-threatening condition that can progress to sepsis and septic shock, leading to significant mortality in the emergency department (ED). The standard diagnostic method, blood culture, is time-consuming and prone to false positives and false negatives. Although...
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2024
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إضافة وسم
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| _version_ | 1852025464375214080 |
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| author | Enrique Hernández-Jiménez (559505) |
| author2 | Erika P. Plata-Menchaca (20102469) Damaris Berbel (20102472) Guillem López de Egea (20102475) Macarena Dastis-Arias (7587032) Laura García-Tejada (20102478) Fabrizio Sbraga (20102481) Pierre Malchair (12410313) Nadia García Muñoz (20102484) Alejandra Larrad Blasco (20102487) Eva Molina Ramírez (20102490) Xose Pérez Fernández (20102493) Joan Sabater Riera (15295586) Arnau Ulsamer (20102496) |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Enrique Hernández-Jiménez (559505) Erika P. Plata-Menchaca (20102469) Damaris Berbel (20102472) Guillem López de Egea (20102475) Macarena Dastis-Arias (7587032) Laura García-Tejada (20102478) Fabrizio Sbraga (20102481) Pierre Malchair (12410313) Nadia García Muñoz (20102484) Alejandra Larrad Blasco (20102487) Eva Molina Ramírez (20102490) Xose Pérez Fernández (20102493) Joan Sabater Riera (15295586) Arnau Ulsamer (20102496) |
| author_role | author |
| dc.creator.none.fl_str_mv | Enrique Hernández-Jiménez (559505) Erika P. Plata-Menchaca (20102469) Damaris Berbel (20102472) Guillem López de Egea (20102475) Macarena Dastis-Arias (7587032) Laura García-Tejada (20102478) Fabrizio Sbraga (20102481) Pierre Malchair (12410313) Nadia García Muñoz (20102484) Alejandra Larrad Blasco (20102487) Eva Molina Ramírez (20102490) Xose Pérez Fernández (20102493) Joan Sabater Riera (15295586) Arnau Ulsamer (20102496) |
| dc.date.none.fl_str_mv | 2024-11-04T04:02:08Z |
| dc.identifier.none.fl_str_mv | 10.3389/fimmu.2024.1447523.s001 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/DataSheet1_Assessing_sepsis-induced_immunosuppression_to_predict_positive_blood_cultures_pdf/27600822 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Immunology Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology bacteremia sepsis endotoxin tolerance immunosuppression blood culture TNFα LPS biomarkers |
| dc.title.none.fl_str_mv | DataSheet1_Assessing sepsis-induced immunosuppression to predict positive blood cultures.pdf |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | Introduction<p>Bacteremia is a life-threatening condition that can progress to sepsis and septic shock, leading to significant mortality in the emergency department (ED). The standard diagnostic method, blood culture, is time-consuming and prone to false positives and false negatives. Although not widely accepted, several clinical and artificial intelligence-based algorithms have been recently developed to predict bacteremia. However, these strategies require further identification of new variables to improve their diagnostic accuracy. This study proposes a novel strategy to predict positive blood cultures by assessing sepsis-induced immunosuppression status through endotoxin tolerance assessment.</p>Methods<p>Optimal assay conditions have been explored and tested in sepsis-suspected patients meeting the Sepsis-3 criteria. Blood samples were collected at ED admission, and endotoxin (lipopolysaccharide, LPS) challenge was performed to evaluate the innate immune response through cytokine profiling.</p>Results<p>Clinical variables, immune cell population biomarkers, and cytokine levels (tumor necrosis factor [TNFα], IL-1β, IL-6, IL-8, and IL-10) were measured. Patients with positive blood cultures exhibited significantly lower TNFα production after LPS challenge than did those with negative blood cultures. The study also included a validation cohort to confirm that the response was consistent.</p>Discussion<p>The results of this study highlight the innate immune system immunosuppression state as a critical parameter for sepsis diagnosis. Notably, the present study identified a reduction in monocyte populations and specific cytokine profiles as potential predictive markers. This study showed that the LPS challenge can be used to effectively distinguish between patients with bloodstream infection leading to sepsis and those whose blood cultures are negative, providinga rapid and reliable diagnostic tool to predict positive blood cultures. The potential applicability of these findings could enhance clinical practice in terms of the accuracy and promptness of sepsis diagnosis in the ED, improving patient outcomes through timely and appropriate treatment.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_cf799e9f13a27f80ddda46bbbed0d262 |
| identifier_str_mv | 10.3389/fimmu.2024.1447523.s001 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/27600822 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | DataSheet1_Assessing sepsis-induced immunosuppression to predict positive blood cultures.pdfEnrique Hernández-Jiménez (559505)Erika P. Plata-Menchaca (20102469)Damaris Berbel (20102472)Guillem López de Egea (20102475)Macarena Dastis-Arias (7587032)Laura García-Tejada (20102478)Fabrizio Sbraga (20102481)Pierre Malchair (12410313)Nadia García Muñoz (20102484)Alejandra Larrad Blasco (20102487)Eva Molina Ramírez (20102490)Xose Pérez Fernández (20102493)Joan Sabater Riera (15295586)Arnau Ulsamer (20102496)ImmunologyApplied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies)AutoimmunityCellular ImmunologyHumoural Immunology and ImmunochemistryImmunogenetics (incl. Genetic Immunology)Innate ImmunityTransplantation ImmunologyTumour ImmunologyImmunology not elsewhere classifiedGenetic ImmunologyAnimal ImmunologyVeterinary Immunologybacteremiasepsisendotoxin toleranceimmunosuppressionblood cultureTNFαLPSbiomarkersIntroduction<p>Bacteremia is a life-threatening condition that can progress to sepsis and septic shock, leading to significant mortality in the emergency department (ED). The standard diagnostic method, blood culture, is time-consuming and prone to false positives and false negatives. Although not widely accepted, several clinical and artificial intelligence-based algorithms have been recently developed to predict bacteremia. However, these strategies require further identification of new variables to improve their diagnostic accuracy. This study proposes a novel strategy to predict positive blood cultures by assessing sepsis-induced immunosuppression status through endotoxin tolerance assessment.</p>Methods<p>Optimal assay conditions have been explored and tested in sepsis-suspected patients meeting the Sepsis-3 criteria. Blood samples were collected at ED admission, and endotoxin (lipopolysaccharide, LPS) challenge was performed to evaluate the innate immune response through cytokine profiling.</p>Results<p>Clinical variables, immune cell population biomarkers, and cytokine levels (tumor necrosis factor [TNFα], IL-1β, IL-6, IL-8, and IL-10) were measured. Patients with positive blood cultures exhibited significantly lower TNFα production after LPS challenge than did those with negative blood cultures. The study also included a validation cohort to confirm that the response was consistent.</p>Discussion<p>The results of this study highlight the innate immune system immunosuppression state as a critical parameter for sepsis diagnosis. Notably, the present study identified a reduction in monocyte populations and specific cytokine profiles as potential predictive markers. This study showed that the LPS challenge can be used to effectively distinguish between patients with bloodstream infection leading to sepsis and those whose blood cultures are negative, providinga rapid and reliable diagnostic tool to predict positive blood cultures. The potential applicability of these findings could enhance clinical practice in terms of the accuracy and promptness of sepsis diagnosis in the ED, improving patient outcomes through timely and appropriate treatment.</p>2024-11-04T04:02:08ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fimmu.2024.1447523.s001https://figshare.com/articles/dataset/DataSheet1_Assessing_sepsis-induced_immunosuppression_to_predict_positive_blood_cultures_pdf/27600822CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/276008222024-11-04T04:02:08Z |
| spellingShingle | DataSheet1_Assessing sepsis-induced immunosuppression to predict positive blood cultures.pdf Enrique Hernández-Jiménez (559505) Immunology Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology bacteremia sepsis endotoxin tolerance immunosuppression blood culture TNFα LPS biomarkers |
| status_str | publishedVersion |
| title | DataSheet1_Assessing sepsis-induced immunosuppression to predict positive blood cultures.pdf |
| title_full | DataSheet1_Assessing sepsis-induced immunosuppression to predict positive blood cultures.pdf |
| title_fullStr | DataSheet1_Assessing sepsis-induced immunosuppression to predict positive blood cultures.pdf |
| title_full_unstemmed | DataSheet1_Assessing sepsis-induced immunosuppression to predict positive blood cultures.pdf |
| title_short | DataSheet1_Assessing sepsis-induced immunosuppression to predict positive blood cultures.pdf |
| title_sort | DataSheet1_Assessing sepsis-induced immunosuppression to predict positive blood cultures.pdf |
| topic | Immunology Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology bacteremia sepsis endotoxin tolerance immunosuppression blood culture TNFα LPS biomarkers |