Bioinformatics analysis indicated that SAT1 is involved in ferroptosis.

(A) Analysis of differentially expressed genes in GEO datasets related to OA. (B) Venn diagram of common DEGs among the GSE55457, GSE114007, GSE55235 and GSE206848 datasets. (C) Top significant pathways for overlapped differentially e...

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Wedi'i Gadw mewn:

Manylion Llyfryddiaeth
Prif Awdur:	Jiexiang Yang (19980594) (author)
Awduron Eraill:	Jing Jiang (130103) (author), Jing Wang (6206297) (author), Lin Luo (429475) (author)
Cyhoeddwyd:	2025
Pynciau:	Biochemistry Cell Biology Molecular Biology Cancer prevalent joint disease potential key gene play important roles novel therapeutic strategy medial meniscus ligament experimental results showed cell death characterized associated geo datasets mediated p53 acetylation inhibits p53 acetylation enhancing p53 acetylation intracellular iron accumulation thereby increasing ferroptosis suppressing trim33 expression oa mouse model related gene sat1 xlink "> ferroptosis axis promotes ferroptosis p53 axis xlink "> sat1 promotes related indicators iron overload induce ferroptosis western blot vivo </ vitro </ regulating trim33 oa progresses oa pathogenesis nonapoptotic form lipid peroxidation downregulating trim33 data indicate close association biological functions atdc5 cells analyzing oa
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Crynodeb:	<p><b>(A)</b> Analysis of differentially expressed genes in GEO datasets related to OA. <b>(B)</b> Venn diagram of common DEGs among the GSE55457, GSE114007, GSE55235 and GSE206848 datasets. <b>(C)</b> Top significant pathways for overlapped differentially expressed genes in OA by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. <b>(D-E)</b> Western blot analysis of SAT1 protein expression levels in ATDC5 cells treated with SAT1 wild-type (WT), SAT1 overexpression (OE-SAT1), and SAT1 knockout (KO) constructs. The corresponding quantitative result is presented in <b>(E)</b>. <b>(F)</b> EDU assay of SAT1 WT, KO and OE ATDC5 cells viability over time ± Erastin 5 μM or RSL3 5 μM. <b>(G)</b> Trypan blue staining cell death in SAT1 WT, KO and OE ATDC5 cells over time ± Erastin 5 μM or RSL3 5 μM. Error bars represent ± / + SEM unless indicated otherwise. p < 0.05, p < 0.01, p < 0.001,**p < 0.0001.</p>

Bioinformatics analysis indicated that SAT1 is involved in ferroptosis.

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