<b>Figure 1. </b>Functional landscape of ABL N-Lobe
<p dir="ltr">We employed deep mutational scanning to map the functional landscape of the ABL N-lobe using the IL-3 dependent Ba/F3 cell line. Ba/F3 cells require IL-3 for survival, but this dependence is overcome by active kinases like BCR-ABL, providing a selection system for functi...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2025
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| الموضوعات: | |
| الوسوم: |
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| الملخص: | <p dir="ltr">We employed deep mutational scanning to map the functional landscape of the ABL N-lobe using the IL-3 dependent Ba/F3 cell line. Ba/F3 cells require IL-3 for survival, but this dependence is overcome by active kinases like BCR-ABL, providing a selection system for functional variants.</p><p dir="ltr">A comprehensive BCR-ABL mutant library was introduced into Ba/F3 cells. Following enrichment, the cells were grown for six days without IL-3, selecting for functional BCR-ABL mutants that promote growth factor-independent survival.</p><p dir="ltr">To quantify the effect of each mutation, we sequenced the mutant library before and after IL-3 withdrawal. This comparison allowed us to determine a functional score for each variant, revealing which mutations were deleterious to BCR-ABL's transformative capacity.</p> |
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