Cryo-EM data processing workflow for rEtpA-1C08 complex.

<p>Simplified cryo-EM data processing workflow including A representative example of an aligned and dose-weighted micrograph (lowpass filtered to 5Å), 2D and 3D class averages, and particle counts at each step. <b>B.</b> Angular distribution and Fourier shell correlation plots for...

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Main Author:	Zachary T. Berndsen (9230176) (author)
Other Authors:	Marjahan Akhtar (10800564) (author), Mahima Thapa (19681706) (author), Tim J. Vickers (7962296) (author), Aaron Schmitz (19681709) (author), Jonathan L. Torres (8551344) (author), Sabyasachi Baboo (574971) (author), Pardeep Kumar (604685) (author), Nazia Khatoon (5561138) (author), Alaullah Sheikh (192291) (author), Melissa Hamrick (19681712) (author), Jolene K. Diedrich (1235706) (author), Salvador Martinez-Bartolome (19681715) (author), Patrick T. Garrett (19681718) (author), John R. Yates III (8072333) (author), Jackson S. Turner (4397776) (author), Renee M. Laird (7817261) (author), Frédéric Poly (427531) (author), Chad K. Porter (9721233) (author), Jeffrey Copps (9230170) (author), Ali H. Ellebedy (8977307) (author), Andrew B. Ward (7832180) (author), James M. Fleckenstein (7901579) (author)
Published:	2024
Subjects:	Biochemistry Microbiology Biotechnology Ecology Immunology Infectious Diseases Virology Biological Sciences not elsewhere classified stimulates adaptive immunity infectious diarrhea annually highly immunogenic resulting div >< p adaptive immunity relevant middle income regions em structures revealed effective bacterial adhesion secreted glycoprotein adhesin mabs directly engage escherichia coli </ promote critical interactions host glycan interactions etpa directs etec coli </ host interaction etec adhesion determined structures decoy regions e </ vaccinated mice toxin delivery terminal domains significantly increased repeat modules mutated etpa monoclonal antibodies intestinal epithelia inhibited etpa general understanding experimental challenge etpa complexes electron microscopy diarrheal severity critical enterotoxigenic cause hundreds blood group
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Cryo-EM data processing workflow for rEtpA-1C08 complex.

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