Table 2_Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.docx
<p>Long-term survival after lung transplantation is limited due to chronic lung allograft dysfunction (CLAD), which encompasses two main phenotypes: bronchiolitis obliterans syndrome (BOS) and restrictive allograft syndrome (RAS). Donor-derived cell-free DNA (dd-cfDNA) is a biomarker for (sub)...
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2024
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| _version_ | 1852024184747589632 |
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| author | H. Beeckmans (20466704) |
| author2 | A. Pagliazzi (20466707) P. Kerckhof (20466710) R. Hofkens (20466713) F. Debackere (20466716) A. Zajacova (20466719) S. Bos (20466722) B. M. Vanaudenaerde (20466725) H. de Loor (20466728) M. Naesens (7487759) R. Vos (6642728) |
| author2_role | author author author author author author author author author author |
| author_facet | H. Beeckmans (20466704) A. Pagliazzi (20466707) P. Kerckhof (20466710) R. Hofkens (20466713) F. Debackere (20466716) A. Zajacova (20466719) S. Bos (20466722) B. M. Vanaudenaerde (20466725) H. de Loor (20466728) M. Naesens (7487759) R. Vos (6642728) |
| author_role | author |
| dc.creator.none.fl_str_mv | H. Beeckmans (20466704) A. Pagliazzi (20466707) P. Kerckhof (20466710) R. Hofkens (20466713) F. Debackere (20466716) A. Zajacova (20466719) S. Bos (20466722) B. M. Vanaudenaerde (20466725) H. de Loor (20466728) M. Naesens (7487759) R. Vos (6642728) |
| dc.date.none.fl_str_mv | 2024-12-23T06:36:09Z |
| dc.identifier.none.fl_str_mv | 10.3389/frtra.2024.1513101.s003 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Table_2_Donor-derived_cell-free_DNA_in_chronic_lung_allograft_dysfunction_phenotypes_a_pilot_study_docx/28080797 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Surgery lung transplantation CLAD dd-cfDNA rejection biomarker |
| dc.title.none.fl_str_mv | Table 2_Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.docx |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <p>Long-term survival after lung transplantation is limited due to chronic lung allograft dysfunction (CLAD), which encompasses two main phenotypes: bronchiolitis obliterans syndrome (BOS) and restrictive allograft syndrome (RAS). Donor-derived cell-free DNA (dd-cfDNA) is a biomarker for (sub)clinical allograft injury and could be a tool for monitoring of lung allograft health across the (pre)clinical spectrum of CLAD. In this proof-of-concept study, we therefore assessed post-transplant plasma dd-cfDNA levels in 20 CLAD patients (11 BOS and 9 RAS) at three consecutive time points free from concurrent infection or acute rejection, during stable condition, preclinical CLAD, and established CLAD (n = 3 × 20 samples). Elevated dd-cfDNA levels were detected in 47% of stable samples, in 66% of preclinical CLAD samples, and in 71% of CLAD samples, indicating ongoing allograft injury. However, dd-cfDNA levels exhibited high intra- and interpatient variability and did not significantly differ between BOS and RAS (p = 0.25), although the range of dd-cfDNA was higher in RAS. Dd-cfDNA detects ongoing allograft injury in patients with CLAD, which warrants further investigation to improve early detection of CLAD.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_d37e1b7912c6dccaa57778db502f2e2f |
| identifier_str_mv | 10.3389/frtra.2024.1513101.s003 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/28080797 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Table 2_Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.docxH. Beeckmans (20466704)A. Pagliazzi (20466707)P. Kerckhof (20466710)R. Hofkens (20466713)F. Debackere (20466716)A. Zajacova (20466719)S. Bos (20466722)B. M. Vanaudenaerde (20466725)H. de Loor (20466728)M. Naesens (7487759)R. Vos (6642728)Surgerylung transplantationCLADdd-cfDNArejectionbiomarker<p>Long-term survival after lung transplantation is limited due to chronic lung allograft dysfunction (CLAD), which encompasses two main phenotypes: bronchiolitis obliterans syndrome (BOS) and restrictive allograft syndrome (RAS). Donor-derived cell-free DNA (dd-cfDNA) is a biomarker for (sub)clinical allograft injury and could be a tool for monitoring of lung allograft health across the (pre)clinical spectrum of CLAD. In this proof-of-concept study, we therefore assessed post-transplant plasma dd-cfDNA levels in 20 CLAD patients (11 BOS and 9 RAS) at three consecutive time points free from concurrent infection or acute rejection, during stable condition, preclinical CLAD, and established CLAD (n = 3 × 20 samples). Elevated dd-cfDNA levels were detected in 47% of stable samples, in 66% of preclinical CLAD samples, and in 71% of CLAD samples, indicating ongoing allograft injury. However, dd-cfDNA levels exhibited high intra- and interpatient variability and did not significantly differ between BOS and RAS (p = 0.25), although the range of dd-cfDNA was higher in RAS. Dd-cfDNA detects ongoing allograft injury in patients with CLAD, which warrants further investigation to improve early detection of CLAD.</p>2024-12-23T06:36:09ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/frtra.2024.1513101.s003https://figshare.com/articles/dataset/Table_2_Donor-derived_cell-free_DNA_in_chronic_lung_allograft_dysfunction_phenotypes_a_pilot_study_docx/28080797CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/280807972024-12-23T06:36:09Z |
| spellingShingle | Table 2_Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.docx H. Beeckmans (20466704) Surgery lung transplantation CLAD dd-cfDNA rejection biomarker |
| status_str | publishedVersion |
| title | Table 2_Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.docx |
| title_full | Table 2_Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.docx |
| title_fullStr | Table 2_Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.docx |
| title_full_unstemmed | Table 2_Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.docx |
| title_short | Table 2_Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.docx |
| title_sort | Table 2_Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.docx |
| topic | Surgery lung transplantation CLAD dd-cfDNA rejection biomarker |