Growth curve of M. smegmatis PLJR962-eccD3-gRNA strain with ivermectin.
<p>Growth curves of <i>M. smegmatis mc</i><sup><i>2</i></sup> 155 wild type strain (diamonds, inner graph (<b>a)</b> and (c), <i>M. smegmatis</i> PLJR962-<i>eccD3</i>-gRNA strain (circles) (<b>a)</b> and (b), a...
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2025
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| Summary: | <p>Growth curves of <i>M. smegmatis mc</i><sup><i>2</i></sup> 155 wild type strain (diamonds, inner graph (<b>a)</b> and (c), <i>M. smegmatis</i> PLJR962-<i>eccD3</i>-gRNA strain (circles) (<b>a)</b> and (b), and <i>M. smegmatis</i> PLJR962-control-gRNA strain (triangles) (c) and (d) without ATc (gray line) and with ATc (black line) 0.0002 μM (100 ng/mL), and with a final concentration of ivermectin of 0.062 μM 64 μg/mL) (a) and (c) and 0.031 μM (32 μg/mL) (b) and (d), and 0.03 μM (20 μg/mL) of kanamycin. In the two ivermectin concentrations tested in <i>M. smegmatis</i> PLJR962-<i>eccD3</i>-gRNA strain, a significant difference was observed among treatments with and without ATc from 20 to 27 hours. An unpaired two-tailed Student’s <i>t</i>-test was used and <i>p</i> < 0.001 (***) and <i>p</i> <0.05 (*) were obtained (<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1012936#pcbi.1012936.s019" target="_blank">S7 Table</a> and MDdb Science Gateway at <a href="http://md-db.org" target="_blank">http://md-db.org</a> with entry ID 690005 <i>in-vitro</i> data). <i>M. smegmatis</i> mc<sup>2</sup> 155 wild type growth curve were included in the inner graph. Each assay was performed with technical triplicates showing the average data with the standard deviation as error bars.</p> |
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