Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology.

<p>Images show square-ended bacilli: (A) Isolate AX2015-1270, (B) AX2015-1277A, (C) AX2015-1152, and (D) AX2015-1136. Encapsulation is visible in (E) AX2015-1270, (F) AX2015-1277A, and (G) AX2015-1152, except in (H) AX2015-1136, which lacked a capsule.</p>

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Bibliographic Details
Main Author:	Sunday Ochonu Ochai (20309058) (author)
Other Authors:	Ayesha Hassim (6714677) (author), Edgar H. Dekker (12083159) (author), Thuto Magome (20309061) (author), Kgaugelo Edward Lekota (6714674) (author), S. Marcus Makgabo (20391356) (author), Lin-Mari de Klerk-Loris (20309067) (author), Louis O. van Schalkwyk (20309070) (author), Pauline L. Kamath (10809397) (author), Wendy C. Turner (12083162) (author), Henriette van Heerden (4804185) (author)
Published:	2024
Subjects:	Medicine Microbiology Cell Biology Biotechnology Cancer Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified zoonotic disease caused pxo1 ), capsule protective antigen (< primer set displayed kruger national park closely related species 2012 – 2020 diseases like anthrax systematically combining microscopy div >< p various marker combinations molecular diagnostic tools 25 positive controls anthrax involves microscopy bacillus anthracis </ accurate anthrax diagnosis combination showed 100 anthrax </ p genetic markers used 1708 grew bacteria >, bapa +< using blood smears genetic markers bapa +< anthracis </ diagnostic samples demonstrated 100 blood smears blood smear clusters using priestia </ peribacillus </ paga </ lef </ gyrb </ cereus </ c </ wildlife mortalities using ba used directly tested positive study identified stored smears southern africa south africa significantly enhances poor survival molecular detection merely 4 likely due faster identification conventional diagnosis comparing microbiological bacterial colonies associated biorisks 890 samples 6 %. 2 %)
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Summary:	<p>Images show square-ended bacilli: (A) Isolate AX2015-1270, (B) AX2015-1277A, (C) AX2015-1152, and (D) AX2015-1136. Encapsulation is visible in (E) AX2015-1270, (F) AX2015-1277A, and (G) AX2015-1152, except in (H) AX2015-1136, which lacked a capsule.</p>

Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology.

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