Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology.
<p>Images show square-ended bacilli: (A) Isolate AX2015-1270, (B) AX2015-1277A, (C) AX2015-1152, and (D) AX2015-1136. Encapsulation is visible in (E) AX2015-1270, (F) AX2015-1277A, and (G) AX2015-1152, except in (H) AX2015-1136, which lacked a capsule.</p>
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , |
| منشور في: |
2024
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| _version_ | 1852024998058786816 |
|---|---|
| author | Sunday Ochonu Ochai (20309058) |
| author2 | Ayesha Hassim (6714677) Edgar H. Dekker (12083159) Thuto Magome (20309061) Kgaugelo Edward Lekota (6714674) S. Marcus Makgabo (20391356) Lin-Mari de Klerk-Loris (20309067) Louis O. van Schalkwyk (20309070) Pauline L. Kamath (10809397) Wendy C. Turner (12083162) Henriette van Heerden (4804185) |
| author2_role | author author author author author author author author author author |
| author_facet | Sunday Ochonu Ochai (20309058) Ayesha Hassim (6714677) Edgar H. Dekker (12083159) Thuto Magome (20309061) Kgaugelo Edward Lekota (6714674) S. Marcus Makgabo (20391356) Lin-Mari de Klerk-Loris (20309067) Louis O. van Schalkwyk (20309070) Pauline L. Kamath (10809397) Wendy C. Turner (12083162) Henriette van Heerden (4804185) |
| author_role | author |
| dc.creator.none.fl_str_mv | Sunday Ochonu Ochai (20309058) Ayesha Hassim (6714677) Edgar H. Dekker (12083159) Thuto Magome (20309061) Kgaugelo Edward Lekota (6714674) S. Marcus Makgabo (20391356) Lin-Mari de Klerk-Loris (20309067) Louis O. van Schalkwyk (20309070) Pauline L. Kamath (10809397) Wendy C. Turner (12083162) Henriette van Heerden (4804185) |
| dc.date.none.fl_str_mv | 2024-11-21T19:02:54Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pntd.0012122.g002 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Microscopic_examination_of_Gram_and_polychrome_methylene_blue-stained_cultures_from_bacterial_isolates_collected_from_wildlife_blood_smears_in_Kruger_National_Park_South_Africa_identified_i_Bacillus_anthracis_i_based_on_morphology_/27885240 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Medicine Microbiology Cell Biology Biotechnology Cancer Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified zoonotic disease caused pxo1 ), capsule protective antigen (< primer set displayed kruger national park closely related species 2012 – 2020 diseases like anthrax systematically combining microscopy div >< p various marker combinations molecular diagnostic tools 25 positive controls anthrax involves microscopy bacillus anthracis </ accurate anthrax diagnosis combination showed 100 anthrax </ p genetic markers used 1708 grew bacteria >, bapa +< using blood smears genetic markers bapa +< anthracis </ diagnostic samples demonstrated 100 blood smears blood smear clusters using priestia </ peribacillus </ paga </ lef </ gyrb </ cereus </ c </ wildlife mortalities using ba used directly tested positive study identified stored smears southern africa south africa significantly enhances poor survival molecular detection merely 4 likely due faster identification conventional diagnosis comparing microbiological bacterial colonies associated biorisks 890 samples 6 %. 2 %) |
| dc.title.none.fl_str_mv | Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Images show square-ended bacilli: (A) Isolate AX2015-1270, (B) AX2015-1277A, (C) AX2015-1152, and (D) AX2015-1136. Encapsulation is visible in (E) AX2015-1270, (F) AX2015-1277A, and (G) AX2015-1152, except in (H) AX2015-1136, which lacked a capsule.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_d5ba544a438679d816bbf3db57dbdb4e |
| identifier_str_mv | 10.1371/journal.pntd.0012122.g002 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/27885240 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology.Sunday Ochonu Ochai (20309058)Ayesha Hassim (6714677)Edgar H. Dekker (12083159)Thuto Magome (20309061)Kgaugelo Edward Lekota (6714674)S. Marcus Makgabo (20391356)Lin-Mari de Klerk-Loris (20309067)Louis O. van Schalkwyk (20309070)Pauline L. Kamath (10809397)Wendy C. Turner (12083162)Henriette van Heerden (4804185)MedicineMicrobiologyCell BiologyBiotechnologyCancerInfectious DiseasesEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedzoonotic disease causedpxo1 ), capsuleprotective antigen (<primer set displayedkruger national parkclosely related species2012 – 2020diseases like anthraxsystematically combining microscopydiv >< pvarious marker combinationsmolecular diagnostic tools25 positive controlsanthrax involves microscopybacillus anthracis </accurate anthrax diagnosiscombination showed 100anthrax </ pgenetic markers used1708 grew bacteria>, bapa +<using blood smearsgenetic markersbapa +<anthracis </diagnostic samplesdemonstrated 100blood smearsblood smearclusters usingpriestia </peribacillus </paga </lef </gyrb </cereus </c </wildlife mortalitiesusing baused directlytested positivestudy identifiedstored smearssouthern africasouth africasignificantly enhancespoor survivalmolecular detectionmerely 4likely duefaster identificationconventional diagnosiscomparing microbiologicalbacterial coloniesassociated biorisks890 samples6 %.2 %)<p>Images show square-ended bacilli: (A) Isolate AX2015-1270, (B) AX2015-1277A, (C) AX2015-1152, and (D) AX2015-1136. Encapsulation is visible in (E) AX2015-1270, (F) AX2015-1277A, and (G) AX2015-1152, except in (H) AX2015-1136, which lacked a capsule.</p>2024-11-21T19:02:54ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pntd.0012122.g002https://figshare.com/articles/figure/Microscopic_examination_of_Gram_and_polychrome_methylene_blue-stained_cultures_from_bacterial_isolates_collected_from_wildlife_blood_smears_in_Kruger_National_Park_South_Africa_identified_i_Bacillus_anthracis_i_based_on_morphology_/27885240CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/278852402024-11-21T19:02:54Z |
| spellingShingle | Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology. Sunday Ochonu Ochai (20309058) Medicine Microbiology Cell Biology Biotechnology Cancer Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified zoonotic disease caused pxo1 ), capsule protective antigen (< primer set displayed kruger national park closely related species 2012 – 2020 diseases like anthrax systematically combining microscopy div >< p various marker combinations molecular diagnostic tools 25 positive controls anthrax involves microscopy bacillus anthracis </ accurate anthrax diagnosis combination showed 100 anthrax </ p genetic markers used 1708 grew bacteria >, bapa +< using blood smears genetic markers bapa +< anthracis </ diagnostic samples demonstrated 100 blood smears blood smear clusters using priestia </ peribacillus </ paga </ lef </ gyrb </ cereus </ c </ wildlife mortalities using ba used directly tested positive study identified stored smears southern africa south africa significantly enhances poor survival molecular detection merely 4 likely due faster identification conventional diagnosis comparing microbiological bacterial colonies associated biorisks 890 samples 6 %. 2 %) |
| status_str | publishedVersion |
| title | Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology. |
| title_full | Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology. |
| title_fullStr | Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology. |
| title_full_unstemmed | Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology. |
| title_short | Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology. |
| title_sort | Microscopic examination of Gram and polychrome methylene blue-stained cultures from bacterial isolates collected from wildlife blood smears in Kruger National Park, South Africa, identified <i>Bacillus anthracis</i> based on morphology. |
| topic | Medicine Microbiology Cell Biology Biotechnology Cancer Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified zoonotic disease caused pxo1 ), capsule protective antigen (< primer set displayed kruger national park closely related species 2012 – 2020 diseases like anthrax systematically combining microscopy div >< p various marker combinations molecular diagnostic tools 25 positive controls anthrax involves microscopy bacillus anthracis </ accurate anthrax diagnosis combination showed 100 anthrax </ p genetic markers used 1708 grew bacteria >, bapa +< using blood smears genetic markers bapa +< anthracis </ diagnostic samples demonstrated 100 blood smears blood smear clusters using priestia </ peribacillus </ paga </ lef </ gyrb </ cereus </ c </ wildlife mortalities using ba used directly tested positive study identified stored smears southern africa south africa significantly enhances poor survival molecular detection merely 4 likely due faster identification conventional diagnosis comparing microbiological bacterial colonies associated biorisks 890 samples 6 %. 2 %) |