Image3_Pan-cancer analysis and experimental validation of FPR3 as a prognostic and immune infiltration-related biomarker for glioma.TIF
<p>Formyl peptide receptor 3 (FPR3) is known to have implications in the progression of various cancer types. Despite this, its biological significance within pan-cancer datasets has yet to be investigated. In this investigation, we scrutinized FPR3’s expression profiles, genetic alterations,...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , , , |
| Published: |
2024
|
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1852026072028151808 |
|---|---|
| author | Chenglin Ye (8277828) |
| author2 | Peng Li (69518) Boxu Chen (17627479) Yong Mo (19822356) Qianrong Huang (19822359) Qiuyun Li (105257) Qinhan Hou (19822362) Ligen Mo (10931754) Jun Yan (28467) |
| author2_role | author author author author author author author author |
| author_facet | Chenglin Ye (8277828) Peng Li (69518) Boxu Chen (17627479) Yong Mo (19822356) Qianrong Huang (19822359) Qiuyun Li (105257) Qinhan Hou (19822362) Ligen Mo (10931754) Jun Yan (28467) |
| author_role | author |
| dc.creator.none.fl_str_mv | Chenglin Ye (8277828) Peng Li (69518) Boxu Chen (17627479) Yong Mo (19822356) Qianrong Huang (19822359) Qiuyun Li (105257) Qinhan Hou (19822362) Ligen Mo (10931754) Jun Yan (28467) |
| dc.date.none.fl_str_mv | 2024-10-09T04:11:51Z |
| dc.identifier.none.fl_str_mv | 10.3389/fgene.2024.1466617.s003 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Image3_Pan-cancer_analysis_and_experimental_validation_of_FPR3_as_a_prognostic_and_immune_infiltration-related_biomarker_for_glioma_TIF/27192189 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy FPR3 pan-cancer glioma biomarker immune infiltration |
| dc.title.none.fl_str_mv | Image3_Pan-cancer analysis and experimental validation of FPR3 as a prognostic and immune infiltration-related biomarker for glioma.TIF |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Formyl peptide receptor 3 (FPR3) is known to have implications in the progression of various cancer types. Despite this, its biological significance within pan-cancer datasets has yet to be investigated. In this investigation, we scrutinized FPR3’s expression profiles, genetic alterations, prognostic significance, immune-related characteristics, methylation status, tumor mutation burden (TMB), and microsatellite instability (MSI) across different types of cancer. We utilized TISCH’s single-cell data to identify immune cells closely associated with FPR3. The predictive significance of FPR3 was evaluated independently in gliomas using data from TCGA and CGGA datasets, leading to the development of a prognostic nomogram. Immunohistochemistry and Western blot analysis confirmed FPR3 expression in gliomas. Lastly, the CCK-8 and wound-healing assays were employed to assess the impact of FPR3 on the proliferation and metastasis of GBM cell lines. In numerous cancer types, heightened FPR3 expression correlated with adverse outcomes, immune cell infiltration, immune checkpoints, TMB, and MSI. In glioma, FPR3 emerged as a notable risk factor, with the prognostic model effectively forecasting patient results. The potential biological relevance of FPR3 was confirmed in glioma, and it was shown to have significant involvement in the processes of glioma growth, immune infiltration, and metastasis. Our results imply a potential association of FPR3 with tumor immunity, indicating its viability as a prognostic indicator in glioma.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_d89dd8df3a8ce4f4d2bcf47bbdce3a2c |
| identifier_str_mv | 10.3389/fgene.2024.1466617.s003 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/27192189 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Image3_Pan-cancer analysis and experimental validation of FPR3 as a prognostic and immune infiltration-related biomarker for glioma.TIFChenglin Ye (8277828)Peng Li (69518)Boxu Chen (17627479)Yong Mo (19822356)Qianrong Huang (19822359)Qiuyun Li (105257)Qinhan Hou (19822362)Ligen Mo (10931754)Jun Yan (28467)GeneticsGenetic EngineeringBiomarkersDevelopmental Genetics (incl. Sex Determination)Epigenetics (incl. Genome Methylation and Epigenomics)Gene Expression (incl. Microarray and other genome-wide approaches)Genome Structure and RegulationGenomicsGenetically Modified AnimalsLivestock CloningGene and Molecular TherapyFPR3pan-cancergliomabiomarkerimmune infiltration<p>Formyl peptide receptor 3 (FPR3) is known to have implications in the progression of various cancer types. Despite this, its biological significance within pan-cancer datasets has yet to be investigated. In this investigation, we scrutinized FPR3’s expression profiles, genetic alterations, prognostic significance, immune-related characteristics, methylation status, tumor mutation burden (TMB), and microsatellite instability (MSI) across different types of cancer. We utilized TISCH’s single-cell data to identify immune cells closely associated with FPR3. The predictive significance of FPR3 was evaluated independently in gliomas using data from TCGA and CGGA datasets, leading to the development of a prognostic nomogram. Immunohistochemistry and Western blot analysis confirmed FPR3 expression in gliomas. Lastly, the CCK-8 and wound-healing assays were employed to assess the impact of FPR3 on the proliferation and metastasis of GBM cell lines. In numerous cancer types, heightened FPR3 expression correlated with adverse outcomes, immune cell infiltration, immune checkpoints, TMB, and MSI. In glioma, FPR3 emerged as a notable risk factor, with the prognostic model effectively forecasting patient results. The potential biological relevance of FPR3 was confirmed in glioma, and it was shown to have significant involvement in the processes of glioma growth, immune infiltration, and metastasis. Our results imply a potential association of FPR3 with tumor immunity, indicating its viability as a prognostic indicator in glioma.</p>2024-10-09T04:11:51ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.3389/fgene.2024.1466617.s003https://figshare.com/articles/figure/Image3_Pan-cancer_analysis_and_experimental_validation_of_FPR3_as_a_prognostic_and_immune_infiltration-related_biomarker_for_glioma_TIF/27192189CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/271921892024-10-09T04:11:51Z |
| spellingShingle | Image3_Pan-cancer analysis and experimental validation of FPR3 as a prognostic and immune infiltration-related biomarker for glioma.TIF Chenglin Ye (8277828) Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy FPR3 pan-cancer glioma biomarker immune infiltration |
| status_str | publishedVersion |
| title | Image3_Pan-cancer analysis and experimental validation of FPR3 as a prognostic and immune infiltration-related biomarker for glioma.TIF |
| title_full | Image3_Pan-cancer analysis and experimental validation of FPR3 as a prognostic and immune infiltration-related biomarker for glioma.TIF |
| title_fullStr | Image3_Pan-cancer analysis and experimental validation of FPR3 as a prognostic and immune infiltration-related biomarker for glioma.TIF |
| title_full_unstemmed | Image3_Pan-cancer analysis and experimental validation of FPR3 as a prognostic and immune infiltration-related biomarker for glioma.TIF |
| title_short | Image3_Pan-cancer analysis and experimental validation of FPR3 as a prognostic and immune infiltration-related biomarker for glioma.TIF |
| title_sort | Image3_Pan-cancer analysis and experimental validation of FPR3 as a prognostic and immune infiltration-related biomarker for glioma.TIF |
| topic | Genetics Genetic Engineering Biomarkers Developmental Genetics (incl. Sex Determination) Epigenetics (incl. Genome Methylation and Epigenomics) Gene Expression (incl. Microarray and other genome-wide approaches) Genome Structure and Regulation Genomics Genetically Modified Animals Livestock Cloning Gene and Molecular Therapy FPR3 pan-cancer glioma biomarker immune infiltration |