Figure 1 from Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival
<p>Knocking down SIRT3 caused ATF4 signaling inhibition but not HIF1a. <b>A,</b> Dendrograms from hierarchical clustering of RNA-seq data from three DLBCL cells lines transduced with lentiviruses containing control (scramble) or two SIRT3 shRNAs. <b>B,</b> Heatmap showi...
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2025
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| 总结: | <p>Knocking down SIRT3 caused ATF4 signaling inhibition but not HIF1a. <b>A,</b> Dendrograms from hierarchical clustering of RNA-seq data from three DLBCL cells lines transduced with lentiviruses containing control (scramble) or two SIRT3 shRNAs. <b>B,</b> Heatmap showing differential expression in SIRT3 knockdown cells versus control (FC > 1.5, <i>q</i> < 0.05). <b>C,</b> Heatmap showing enrichment of SIRT3 knockdown signatures within key pathways. CHIP, chromatin immunoprecipitation; CHOP, C/EBP homologous protein; dn, down; KEGG, Kyoto Encyclopedia of Genes and Genomes; MEF, mouse embryonic fibroblast; TM, tunicamycin. <b>D,</b> GSEA (<a href="#bib51 bib52" target="_blank">51, 52</a>) showing the enrichment of ATF4 target genes in SIRT3-downregulated genes in Karpas 422, OCI-LY1, and HBL1 cells with SIRT3 sh1 versus control scramble shRNAs. The rank lists were from RNA-seq analysis from <b>B</b>. ATF4 target genes were summarized from previous publications (<a href="#bib17 bib18" target="_blank">17, 18</a>). <b>E,</b> GSEA (<a href="#bib51 bib52" target="_blank">51, 52</a>) showing the enrichment of ATF4 target genes in SIRT3-downregulated genes in Karpas 422, OCI-LY1, and HBL1 cells with SIRT3 sh2 versus control scramble shRNAs. The rank lists were from RNA-seq analysis from <b>B</b>. The same ATF4 target gene list was used here as in <b>D</b>.</p> |
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