Infection dynamics and phylogenetics of HEV in two farms.

<p><b>2A:</b> Farm structure, indicating all compartments in black and white, and the sampled farm compartments by three colored rectangles, each with twelve pens inside. <b>2B</b>: Timeline of the study with three sampled batches and weekly HEV PCR results per pen (hor...

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Glavni autor: Marina Meester (11765542) (author)
Daljnji autori: Cecilia Valenzuela Agüí (22676709) (author), Tijs J. Tobias (9582206) (author), Claire Guinat (3184698) (author), Martijn Bouwknegt (2620273) (author), Louis du Plessis (3264087) (author), Egil A.J. Fischer (19205508) (author), Mirlin Spaninks (4405027) (author), Tanja Stadler (118822) (author), Wim H.M. van der Poel (11765557) (author), Arjan Stegeman (199275) (author)
Izdano: 2025
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_version_ 1849927641261408256
author Marina Meester (11765542)
author2 Cecilia Valenzuela Agüí (22676709)
Tijs J. Tobias (9582206)
Claire Guinat (3184698)
Martijn Bouwknegt (2620273)
Louis du Plessis (3264087)
Egil A.J. Fischer (19205508)
Mirlin Spaninks (4405027)
Tanja Stadler (118822)
Wim H.M. van der Poel (11765557)
Arjan Stegeman (199275)
author2_role author
author
author
author
author
author
author
author
author
author
author_facet Marina Meester (11765542)
Cecilia Valenzuela Agüí (22676709)
Tijs J. Tobias (9582206)
Claire Guinat (3184698)
Martijn Bouwknegt (2620273)
Louis du Plessis (3264087)
Egil A.J. Fischer (19205508)
Mirlin Spaninks (4405027)
Tanja Stadler (118822)
Wim H.M. van der Poel (11765557)
Arjan Stegeman (199275)
author_role author
dc.creator.none.fl_str_mv Marina Meester (11765542)
Cecilia Valenzuela Agüí (22676709)
Tijs J. Tobias (9582206)
Claire Guinat (3184698)
Martijn Bouwknegt (2620273)
Louis du Plessis (3264087)
Egil A.J. Fischer (19205508)
Mirlin Spaninks (4405027)
Tanja Stadler (118822)
Wim H.M. van der Poel (11765557)
Arjan Stegeman (199275)
dc.date.none.fl_str_mv 2025-11-24T18:35:09Z
dc.identifier.none.fl_str_mv 10.1371/journal.ppat.1013710.g002
dc.relation.none.fl_str_mv https://figshare.com/articles/figure/Infection_dynamics_and_phylogenetics_of_HEV_in_two_farms_/30697764
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biotechnology
Ecology
Cancer
Science Policy
Infectious Diseases
Virology
Biological Sciences not elsewhere classified
retrieving 89 near
effective reproduction number
eating contaminated pork
consecutively housed groups
observed phylogenetic clustering
integrated epidemiological data
making hev control
hev &# 8217
sequenced per batch
pig farms crucial
many pig farms
zoonotic risk may
pigs housed separately
hpd interval 1
hepatitis e virus
div >< p
3 &# 8211
pens tested positive
pens per compartment
investigated hev transmission
hev transmission dynamics
zoonotic virus
phylogenetic data
batch may
causes hepatitis
remained hev
hev persists
one batch
pens within
transmission routes
suggesting transmission
quantify transmission
pen transmission
enabling transmission
public health
positive pen
pigs separated
pigs ).
nine months
negative thanks
genotype 3
findings show
findings reveal
environmental routes
become infected
7 ).
dc.title.none.fl_str_mv Infection dynamics and phylogenetics of HEV in two farms.
dc.type.none.fl_str_mv Image
Figure
info:eu-repo/semantics/publishedVersion
image
description <p><b>2A:</b> Farm structure, indicating all compartments in black and white, and the sampled farm compartments by three colored rectangles, each with twelve pens inside. <b>2B</b>: Timeline of the study with three sampled batches and weekly HEV PCR results per pen (horizontal line) per compartment (same colors as 2A). Legend: Serological results at the start and end of batch B and C are indicated by pink circles that are darker in case more pigs were HEV seropositive (maximally 4). Open circles (fecal dropping samples) and triangles (boot sock samples) indicate weeks in which HEV sequence data was obtained. <b>2C</b>: Maximum likelihood tree with all sequences from both farms, and HEV 3c sequences extracted from NCBI. Legend: Tip labels represent the farm (A or <b>B)</b>, compartment (1/2/3), batch (A/B/C), week of sampling within the batch, type of sample (fecal dropping (FD) or bootsock (BS) and pen number (1 to 36). Numbers at the internal nodes represent bootstrap support values (>0.70).</p>
eu_rights_str_mv openAccess
id Manara_de2f71e784f9e40228f2100e246b61fd
identifier_str_mv 10.1371/journal.ppat.1013710.g002
network_acronym_str Manara
network_name_str ManaraRepo
oai_identifier_str oai:figshare.com:article/30697764
publishDate 2025
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv CC BY 4.0
spelling Infection dynamics and phylogenetics of HEV in two farms.Marina Meester (11765542)Cecilia Valenzuela Agüí (22676709)Tijs J. Tobias (9582206)Claire Guinat (3184698)Martijn Bouwknegt (2620273)Louis du Plessis (3264087)Egil A.J. Fischer (19205508)Mirlin Spaninks (4405027)Tanja Stadler (118822)Wim H.M. van der Poel (11765557)Arjan Stegeman (199275)BiotechnologyEcologyCancerScience PolicyInfectious DiseasesVirologyBiological Sciences not elsewhere classifiedretrieving 89 neareffective reproduction numbereating contaminated porkconsecutively housed groupsobserved phylogenetic clusteringintegrated epidemiological datamaking hev controlhev &# 8217sequenced per batchpig farms crucialmany pig farmszoonotic risk maypigs housed separatelyhpd interval 1hepatitis e virusdiv >< p3 &# 8211pens tested positivepens per compartmentinvestigated hev transmissionhev transmission dynamicszoonotic virusphylogenetic databatch maycauses hepatitisremained hevhev persistsone batchpens withintransmission routessuggesting transmissionquantify transmissionpen transmissionenabling transmissionpublic healthpositive penpigs separatedpigs ).nine monthsnegative thanksgenotype 3findings showfindings revealenvironmental routesbecome infected7 ).<p><b>2A:</b> Farm structure, indicating all compartments in black and white, and the sampled farm compartments by three colored rectangles, each with twelve pens inside. <b>2B</b>: Timeline of the study with three sampled batches and weekly HEV PCR results per pen (horizontal line) per compartment (same colors as 2A). Legend: Serological results at the start and end of batch B and C are indicated by pink circles that are darker in case more pigs were HEV seropositive (maximally 4). Open circles (fecal dropping samples) and triangles (boot sock samples) indicate weeks in which HEV sequence data was obtained. <b>2C</b>: Maximum likelihood tree with all sequences from both farms, and HEV 3c sequences extracted from NCBI. Legend: Tip labels represent the farm (A or <b>B)</b>, compartment (1/2/3), batch (A/B/C), week of sampling within the batch, type of sample (fecal dropping (FD) or bootsock (BS) and pen number (1 to 36). Numbers at the internal nodes represent bootstrap support values (>0.70).</p>2025-11-24T18:35:09ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013710.g002https://figshare.com/articles/figure/Infection_dynamics_and_phylogenetics_of_HEV_in_two_farms_/30697764CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306977642025-11-24T18:35:09Z
spellingShingle Infection dynamics and phylogenetics of HEV in two farms.
Marina Meester (11765542)
Biotechnology
Ecology
Cancer
Science Policy
Infectious Diseases
Virology
Biological Sciences not elsewhere classified
retrieving 89 near
effective reproduction number
eating contaminated pork
consecutively housed groups
observed phylogenetic clustering
integrated epidemiological data
making hev control
hev &# 8217
sequenced per batch
pig farms crucial
many pig farms
zoonotic risk may
pigs housed separately
hpd interval 1
hepatitis e virus
div >< p
3 &# 8211
pens tested positive
pens per compartment
investigated hev transmission
hev transmission dynamics
zoonotic virus
phylogenetic data
batch may
causes hepatitis
remained hev
hev persists
one batch
pens within
transmission routes
suggesting transmission
quantify transmission
pen transmission
enabling transmission
public health
positive pen
pigs separated
pigs ).
nine months
negative thanks
genotype 3
findings show
findings reveal
environmental routes
become infected
7 ).
status_str publishedVersion
title Infection dynamics and phylogenetics of HEV in two farms.
title_full Infection dynamics and phylogenetics of HEV in two farms.
title_fullStr Infection dynamics and phylogenetics of HEV in two farms.
title_full_unstemmed Infection dynamics and phylogenetics of HEV in two farms.
title_short Infection dynamics and phylogenetics of HEV in two farms.
title_sort Infection dynamics and phylogenetics of HEV in two farms.
topic Biotechnology
Ecology
Cancer
Science Policy
Infectious Diseases
Virology
Biological Sciences not elsewhere classified
retrieving 89 near
effective reproduction number
eating contaminated pork
consecutively housed groups
observed phylogenetic clustering
integrated epidemiological data
making hev control
hev &# 8217
sequenced per batch
pig farms crucial
many pig farms
zoonotic risk may
pigs housed separately
hpd interval 1
hepatitis e virus
div >< p
3 &# 8211
pens tested positive
pens per compartment
investigated hev transmission
hev transmission dynamics
zoonotic virus
phylogenetic data
batch may
causes hepatitis
remained hev
hev persists
one batch
pens within
transmission routes
suggesting transmission
quantify transmission
pen transmission
enabling transmission
public health
positive pen
pigs separated
pigs ).
nine months
negative thanks
genotype 3
findings show
findings reveal
environmental routes
become infected
7 ).