Evaluating the Impact of Laser-Induced Shock Waves on <i>Drosophila</i> Brain Tissue: A Study of Morphology and Volume Influence on Tissue Displacement
<p dir="ltr">Laser-induced shock waves (LIS) provide a powerful tool to simulate traumatic brain injury (TBI) in experimental models. This study examined how morphology and volume influence <i>Drosophila</i> brain tissue responses to LIS, offering insights into the biomec...
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2025
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| Summary: | <p dir="ltr">Laser-induced shock waves (LIS) provide a powerful tool to simulate traumatic brain injury (TBI) in experimental models. This study examined how morphology and volume influence <i>Drosophila</i> brain tissue responses to LIS, offering insights into the biomechanics of tissue damage.</p><p dir="ltr">Brain tissues were isolated by removing the eyes and mandibles, then stained with Dead-Red and NucBlue to identify apoptotic and total cell populations. Shock waves were applied at 5 µm steps, progressing through tissues until displacement occurred. Two morphologies were studied: (1) a wider, flatter structure with a larger base area, and (2) a taller, narrower structure. Imaging and volume measurements were performed using a Zeiss Axiovert 200M microscope with controlled environmental conditions.</p><p dir="ltr">Results showed that the wider tissue, with a base area of 194,132.11 µm², required 15 µm of laser depth before displacement, while the narrower tissue, with a base area of 116,924.39 µm², required 25 µm of depth. Despite differences in shape, both tissues exhibited nearly identical displacement volumes (~2.9 × 10⁶ µm³). This indicates that tissue volume, rather than morphology, is the key determinant of resistance to LIS.</p><p dir="ltr">These findings demonstrate that LIS can reproducibly displace brain tissues, providing a consistent experimental platform for studying tissue biomechanics. The observed role of tissue volume in damage resistance has implications for understanding TBI and could inform tissue engineering and regenerative medicine approaches. Further exploration of LIS across different tissue types may expand its utility in mechanobiology.</p> |
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