Differences between the High stability (H) and Low stability (L) groups of patients in cognitive performance.

مواد مشابهة

• Stability of β-catenin variants. (A) COS-7 cells were transfected with plasmids encoding the β-catenin variants (EV, empty vector; WT, wild type) and kept untreated (-) or incubated (+) in the presence of cycloheximide (CHX, 800 μg/ml) for 6 h to monitor protein degradation. The top panel shows a representative immunoblot using sequentially anti-β-catenin N-terminal and C-terminal antibodies. The bottom panel shows the relative quantification of the representative bands from each variant. Bars represent the relative expression after CHX treatment, with regard to the expression under the untreated conditions ± SD, from at least two independent experiments. (B) COS-7 cells were processed as in (A) and kept untreated (0) or incubated in the presence of cycloheximide (CHX, 800 μg/ml) for 2h or 6 h, as indicated. Immunoblots were performed using anti-β-catenin N-terminal or anti-GAPDH antibodies, as indicated. In the left panel,
  حسب: Caroline E. Nunes-Xavier (3607922)
  منشور في: (2025)
• CTNNB1 pathogenic variants analysed in the study.
  حسب: Caroline E. Nunes-Xavier (3607922)
  منشور في: (2025)
• Reconstitution of β-catenin function by translational readthrough. (A) COS-7 cells were transfected with plasmids encoding the β-catenin variants (EV, empty vector; WT, wild type) and kept untreated (-) or incubated (+) in the presence of G418 (200 μg/ml) for 24 h. A representative immunoblot is shown using anti-β-catenin N-terminal or anti-GAPDH antibodies. (B) COS-7 cells were transfected with plasmids encoding the R474* β-catenin variant (EV, empty vector; WT, wild type) and kept untreated (-) or incubated (+) in the presence of G418 (200 μg/ml) and Y-320 (1 μM) for 24 h, as indicated. The top panel shows a representative immunoblot using anti-β-catenin N-terminal or anti-GAPDH antibodies. The bottom panel shows the relative quantification of the β-catenin full-length bands from each variant, from at least two independent experiments. *, p = 0.0133. (C) Transcriptional activity of β-catenin variants. COS-7 cells
  حسب: Caroline E. Nunes-Xavier (3607922)
  منشور في: (2025)
• Reconstitution of β-catenin function by proteasome inhibition. (A) COS-7 cells were transfected with plasmids encoding the β-catenin variants (WT, wild type) and kept untreated (-) or incubated (+) in the presence of MG-132 (10 μM) or cycloheximide (CHX, 800 μg/ml) for 6 h. The left panel shows representative immunoblots using anti-β-catenin N-terminal or anti-GAPDH antibodies. The right panel shows the relative quantification of the bands from each variant, from at least two independent experiments. **, p = 0.0003; ***, p < 0.0001. (B) Transcriptional activity of β-catenin variants. COS-7 cells were transfected with plasmids encoding the indicated β-catenin variants (EV, empty vector; WT, wild type) and kept untreated (Control) or incubated in the presence of MG-132 (10 μM) for 6 h, as indicated. Cells were processed for TCF/LEF-driven luciferase activity, and luminescence is shown in arbitrary units (AU), fr
  حسب: Caroline E. Nunes-Xavier (3607922)
  منشور في: (2025)
• (A) Expression of N-terminal and C-terminal truncated β-catenin proteoforms.
  حسب: Caroline E. Nunes-Xavier (3607922)
  منشور في: (2025)