Table 4_Positional distribution and conservation of major phosphorylated sites in the human kinome.xlsx
<p>The human protein kinome is a group of over 500 therapeutically relevant kinases. Exemplified by over 10,000 phosphorylated sites reported in global phosphoproteomes, kinases are also highly regulated by phosphorylation. Currently, 1008 phosphorylated sites in 273 kinases are associated wit...
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| مؤلفون آخرون: | , , , , , , , , , |
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| _version_ | 1852021435243954176 |
|---|---|
| author | Athira Perunelly Gopalakrishnan (21025124) |
| author2 | Prathik Basthikoppa Shivamurthy (21025127) Mukhtar Ahmed (732942) Samseera Ummar (21025130) Poornima Ramesh (10586884) Sonet Daniel Thomas (21025133) Althaf Mahin (21025136) Mahammad Nisar (21025139) Sowmya Soman (16482733) Yashwanth Subbannayya (2131048) Rajesh Raju (771787) |
| author2_role | author author author author author author author author author author |
| author_facet | Athira Perunelly Gopalakrishnan (21025124) Prathik Basthikoppa Shivamurthy (21025127) Mukhtar Ahmed (732942) Samseera Ummar (21025130) Poornima Ramesh (10586884) Sonet Daniel Thomas (21025133) Althaf Mahin (21025136) Mahammad Nisar (21025139) Sowmya Soman (16482733) Yashwanth Subbannayya (2131048) Rajesh Raju (771787) |
| author_role | author |
| dc.creator.none.fl_str_mv | Athira Perunelly Gopalakrishnan (21025124) Prathik Basthikoppa Shivamurthy (21025127) Mukhtar Ahmed (732942) Samseera Ummar (21025130) Poornima Ramesh (10586884) Sonet Daniel Thomas (21025133) Althaf Mahin (21025136) Mahammad Nisar (21025139) Sowmya Soman (16482733) Yashwanth Subbannayya (2131048) Rajesh Raju (771787) |
| dc.date.none.fl_str_mv | 2025-04-09T07:57:34Z |
| dc.identifier.none.fl_str_mv | 10.3389/fmolb.2025.1557835.s007 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/Table_4_Positional_distribution_and_conservation_of_major_phosphorylated_sites_in_the_human_kinome_xlsx/28758959 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Molecular Biology predominant phosphorylated sites kinome classification kinase domain kinase family functional phosphorylated sites |
| dc.title.none.fl_str_mv | Table 4_Positional distribution and conservation of major phosphorylated sites in the human kinome.xlsx |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <p>The human protein kinome is a group of over 500 therapeutically relevant kinases. Exemplified by over 10,000 phosphorylated sites reported in global phosphoproteomes, kinases are also highly regulated by phosphorylation. Currently, 1008 phosphorylated sites in 273 kinases are associated with their regulation of activation/inhibition, and a few in 30 kinases are associated with altered activity. Phosphorylated sites in 196 kinases are related to other molecular functions such as localization and protein interactions. Over 8,000 phosphorylated sites, including all those in 517 kinases are unassigned to any functions. This imposes a significant bias and challenge for the effective analysis of global phosphoproteomics datasets. Hence, we derived a set of stably and frequently detected phosphorylated sites (representative phosphorylated sites) across diverse experimental conditions annotated in the PhosphoSitePlus database and presumed them to be relevant to the human kinase regulatory network. Analysis of these representative phosphorylated sites led to the classification of 449 kinases into four distinct categories (kinases with phosphorylated sites apportioned (PaKD) and enigmatic (PeKD), and those with predominantly within kinase domain (PiKD) and outside kinase domain (PoKD)). Knowledge-based functional analysis and sequence conservation across the family/subfamily identified phosphorylated sites unique to specific kinases that could contribute to their unique functions. This classification of representative kinase phosphorylated sites enhance our understanding of prioritized validation and provides a novel framework for targeted phosphorylated site enrichment approaches. Phosphorylated sites in kinases associated with dysregulation in diseases were frequently located outside the kinase domain, and suggesting their regulatory roles and opportunities for phosphorylated site-directed therapeutic approaches.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_dfd689d33d222009eae9ee50e480d4e0 |
| identifier_str_mv | 10.3389/fmolb.2025.1557835.s007 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/28758959 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Table 4_Positional distribution and conservation of major phosphorylated sites in the human kinome.xlsxAthira Perunelly Gopalakrishnan (21025124)Prathik Basthikoppa Shivamurthy (21025127)Mukhtar Ahmed (732942)Samseera Ummar (21025130)Poornima Ramesh (10586884)Sonet Daniel Thomas (21025133)Althaf Mahin (21025136)Mahammad Nisar (21025139)Sowmya Soman (16482733)Yashwanth Subbannayya (2131048)Rajesh Raju (771787)Molecular Biologypredominant phosphorylated siteskinome classificationkinase domainkinase familyfunctional phosphorylated sites<p>The human protein kinome is a group of over 500 therapeutically relevant kinases. Exemplified by over 10,000 phosphorylated sites reported in global phosphoproteomes, kinases are also highly regulated by phosphorylation. Currently, 1008 phosphorylated sites in 273 kinases are associated with their regulation of activation/inhibition, and a few in 30 kinases are associated with altered activity. Phosphorylated sites in 196 kinases are related to other molecular functions such as localization and protein interactions. Over 8,000 phosphorylated sites, including all those in 517 kinases are unassigned to any functions. This imposes a significant bias and challenge for the effective analysis of global phosphoproteomics datasets. Hence, we derived a set of stably and frequently detected phosphorylated sites (representative phosphorylated sites) across diverse experimental conditions annotated in the PhosphoSitePlus database and presumed them to be relevant to the human kinase regulatory network. Analysis of these representative phosphorylated sites led to the classification of 449 kinases into four distinct categories (kinases with phosphorylated sites apportioned (PaKD) and enigmatic (PeKD), and those with predominantly within kinase domain (PiKD) and outside kinase domain (PoKD)). Knowledge-based functional analysis and sequence conservation across the family/subfamily identified phosphorylated sites unique to specific kinases that could contribute to their unique functions. This classification of representative kinase phosphorylated sites enhance our understanding of prioritized validation and provides a novel framework for targeted phosphorylated site enrichment approaches. Phosphorylated sites in kinases associated with dysregulation in diseases were frequently located outside the kinase domain, and suggesting their regulatory roles and opportunities for phosphorylated site-directed therapeutic approaches.</p>2025-04-09T07:57:34ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.3389/fmolb.2025.1557835.s007https://figshare.com/articles/dataset/Table_4_Positional_distribution_and_conservation_of_major_phosphorylated_sites_in_the_human_kinome_xlsx/28758959CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/287589592025-04-09T07:57:34Z |
| spellingShingle | Table 4_Positional distribution and conservation of major phosphorylated sites in the human kinome.xlsx Athira Perunelly Gopalakrishnan (21025124) Molecular Biology predominant phosphorylated sites kinome classification kinase domain kinase family functional phosphorylated sites |
| status_str | publishedVersion |
| title | Table 4_Positional distribution and conservation of major phosphorylated sites in the human kinome.xlsx |
| title_full | Table 4_Positional distribution and conservation of major phosphorylated sites in the human kinome.xlsx |
| title_fullStr | Table 4_Positional distribution and conservation of major phosphorylated sites in the human kinome.xlsx |
| title_full_unstemmed | Table 4_Positional distribution and conservation of major phosphorylated sites in the human kinome.xlsx |
| title_short | Table 4_Positional distribution and conservation of major phosphorylated sites in the human kinome.xlsx |
| title_sort | Table 4_Positional distribution and conservation of major phosphorylated sites in the human kinome.xlsx |
| topic | Molecular Biology predominant phosphorylated sites kinome classification kinase domain kinase family functional phosphorylated sites |