Global gene transcript changes caused by ethylene.
<p><i>A. brasilense</i> cells were grown in liquid culture as detailed in the materials and methods for 4 h in the presence or absence of 100 ppb ethylene. Samples were prepared as detailed in the materials and methods and RNA-seq analysis and bioinformatics analyses was carried ou...
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2025
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| Summary: | <p><i>A. brasilense</i> cells were grown in liquid culture as detailed in the materials and methods for 4 h in the presence or absence of 100 ppb ethylene. Samples were prepared as detailed in the materials and methods and RNA-seq analysis and bioinformatics analyses was carried out by GeneWiz (South Plainfield, NJ, USA). <b>A)</b> Heatmap showing relative abundance of each gene in individual control (no ethylene) and ethylene-treated samples. Included are gene transcripts that have a log<sub>2</sub> change ≥ |0.5| with an adjusted <i>p</i>-value ≤ 0.05. <b>B)</b> Pie charts showing the percent of genes downregulated (top) or upregulated (bottom) by ethylene in various Gene Ontology categories using the Kyoto Encyclopedia of Genes (KEGG). <b>C)</b> Volcano plot showing global transcriptional changes caused by ethylene treatment. Each data point represents a gene. Genes with an adjusted <i>p</i>-value ≤ 0.05 and a log2 fold ≥ 1 are indicated by red dots. Genes with an adjusted <i>p</i>-value ≤ 0.05 and as log2 fold change ≤ -1 are indicated by blue dots.</p> |
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