Methylation of RBM39 reverses the inhibitory of Indisulam on NSCLC.

Methylation of RBM39 reverses the inhibitory of Indisulam on NSCLC.

<p>(<b>A</b>) A549 and H460 cells transfected with empty vector, RBM39, or R92K, respectively, were treated with Indisulam, and the protein levels of stem-related markers were analyzed by western blotting. (<b>B</b>) MTT assays were performed to test the cell growth abi...

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Main Author: Tongjia Zhang (16496661) (author)
Other Authors: Shujie Wang (750318) (author), Yue Zhou (71752) (author), Zitao Jiao (21485389) (author), Kejia Lu (7538975) (author), Xinyi Liu (186154) (author), Hui Li (32376) (author), Wei Jiang (14986) (author), Xiaowei Zhang (6505) (author)
Published: 2025
Subjects:
Biophysics
Biochemistry
Cell Biology
Genetics
Molecular Biology
Pharmacology
Biotechnology
Cancer
Hematology
Infectious Diseases
Plant Biology
Biological Sciences not elsewhere classified
xenograft mouse models
remains poorly understood
promoting alternative splicing
promotes alternative splicing
potential therapeutic strategy
binding protein 39
xlink "> indisulam
prmt6 enhances resistance
prmt6 methylates rbm39
methylation inhibits indisulam
oncogenes </ p
inhibiting prmt6
indisulam resistance
acquired resistance
ultimately leads
tumor growth
tumor efficacy
tumor activity
specific target
significantly improves
rbm39 ),
proteasomal degradation
molecular basis
malignant proliferation
line therapy
key biomarker
induced ubiquitination
closely associated
clinical efficacy
based compound
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Summary:<p>(<b>A</b>) A549 and H460 cells transfected with empty vector, RBM39, or R92K, respectively, were treated with Indisulam, and the protein levels of stem-related markers were analyzed by western blotting. (<b>B</b>) MTT assays were performed to test the cell growth ability in the cells above treated with Indisulam. (<b>C</b>) The proportion of CD44<sup>+</sup> CD133<sup>+</sup> cells populations in indicated groups was quantified by flow cytometry. (<b>D)</b> Western blotting was used to detect N-cadherin, vimentin, and E-cadherin levels in A549 and H460 cells transfected with the indicated constructs and treated with Indisulam. (<b>E</b>) Wound healing assays were performed to evaluate the migratory ability of transfected cells after Indisulam exposure. Bars = 100 μm. (<b>F</b>) Transwell assays assessed the migratory and invasive capacities of the indicated cells above, Bars = 200 μm. Data calculates the mean ± SD (<i>n</i> = 3). *<i>p</i> < 0.05, **<i>p</i> < 0.01, ***<i>p</i> < 0.001. Statistical analysis was calculated using the one-way ANOVA. The underlying data for <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002846#pbio.3002846.g006" target="_blank">Fig 6B</a>, <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002846#pbio.3002846.g006" target="_blank">6C</a>, <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002846#pbio.3002846.g006" target="_blank">6E</a>, and <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002846#pbio.3002846.g006" target="_blank">6F</a> can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3002846#pbio.3002846.s013" target="_blank">S3 Data</a>. NSCLC, non–small cell lung cancer; RBM39, RNA-binding protein 39; MTT, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.</p>

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