Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i>
<p>Yeast cells carrying the CYP3201B1 expression plasmid or an empty vector were incubated with PAN. PAN after the reaction was quantified using ultra-performance liquid chromatography. Reactions were carried out in triplicate (<i>n</i> = 3), error bars show the standard deviation...
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2025
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| _version_ | 1849927642407501824 |
|---|---|
| author | Takuya Yamaguchi (4543105) |
| author2 | Yasuhisa Asano (727606) |
| author2_role | author |
| author_facet | Takuya Yamaguchi (4543105) Yasuhisa Asano (727606) |
| author_role | author |
| dc.creator.none.fl_str_mv | Takuya Yamaguchi (4543105) Yasuhisa Asano (727606) |
| dc.date.none.fl_str_mv | 2025-11-24T18:29:58Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pgen.1011955.s014 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Comparison_of_phenylacetonitrile_PAN_metabolizing_activity_of_i_Saccharomyces_cerevisiae_i_ChCR11_and_INVSc1_expressing_i_CYP3201B1_i_/30697191 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Evolutionary Biology Ecology Plant Biology Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified subsequently converting aldoxime natural defensive chemicals indicating sequential duplication catalyse aldoxime dehydration div >< p cyanogenic millipedes evolved chamberlinius hualienensis </ hualienensis </ r </ typically lineage related enzymes plant lineages plant kingdoms paralogous genes millipedes demonstrates mechanisms underlying hydroxynitrile lyase hydrogen cyanide giving rise enzymes independently dependent monooxygenase deeper understanding cytochrome p450 complete set coding genes biological characterisation >)- mandelonitrile |
| dc.title.none.fl_str_mv | Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i> |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Yeast cells carrying the CYP3201B1 expression plasmid or an empty vector were incubated with PAN. PAN after the reaction was quantified using ultra-performance liquid chromatography. Reactions were carried out in triplicate (<i>n</i> = 3), error bars show the standard deviation of the replicate measurements, the error bar centers are the means of the replicate measurements, and the replicate measurements are represented as black dots. Bars labelled with different letters indicate a significant difference (<i>P</i> < 0.05) as determined by Tukey’s honest significant difference test.</p> <p>(TIF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_e6f07e334f069f737cfc4553137feecc |
| identifier_str_mv | 10.1371/journal.pgen.1011955.s014 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697191 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i>Takuya Yamaguchi (4543105)Yasuhisa Asano (727606)BiochemistryEvolutionary BiologyEcologyPlant BiologyVirologyBiological Sciences not elsewhere classifiedChemical Sciences not elsewhere classifiedsubsequently converting aldoximenatural defensive chemicalsindicating sequential duplicationcatalyse aldoxime dehydrationdiv >< pcyanogenic millipedes evolvedchamberlinius hualienensis </hualienensis </r </typically lineagerelated enzymesplant lineagesplant kingdomsparalogous genesmillipedes demonstratesmechanisms underlyinghydroxynitrile lyasehydrogen cyanidegiving riseenzymes independentlydependent monooxygenasedeeper understandingcytochrome p450complete setcoding genesbiological characterisation>)- mandelonitrile<p>Yeast cells carrying the CYP3201B1 expression plasmid or an empty vector were incubated with PAN. PAN after the reaction was quantified using ultra-performance liquid chromatography. Reactions were carried out in triplicate (<i>n</i> = 3), error bars show the standard deviation of the replicate measurements, the error bar centers are the means of the replicate measurements, and the replicate measurements are represented as black dots. Bars labelled with different letters indicate a significant difference (<i>P</i> < 0.05) as determined by Tukey’s honest significant difference test.</p> <p>(TIF)</p>2025-11-24T18:29:58ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pgen.1011955.s014https://figshare.com/articles/figure/Comparison_of_phenylacetonitrile_PAN_metabolizing_activity_of_i_Saccharomyces_cerevisiae_i_ChCR11_and_INVSc1_expressing_i_CYP3201B1_i_/30697191CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306971912025-11-24T18:29:58Z |
| spellingShingle | Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i> Takuya Yamaguchi (4543105) Biochemistry Evolutionary Biology Ecology Plant Biology Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified subsequently converting aldoxime natural defensive chemicals indicating sequential duplication catalyse aldoxime dehydration div >< p cyanogenic millipedes evolved chamberlinius hualienensis </ hualienensis </ r </ typically lineage related enzymes plant lineages plant kingdoms paralogous genes millipedes demonstrates mechanisms underlying hydroxynitrile lyase hydrogen cyanide giving rise enzymes independently dependent monooxygenase deeper understanding cytochrome p450 complete set coding genes biological characterisation >)- mandelonitrile |
| status_str | publishedVersion |
| title | Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i> |
| title_full | Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i> |
| title_fullStr | Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i> |
| title_full_unstemmed | Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i> |
| title_short | Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i> |
| title_sort | Comparison of phenylacetonitrile (PAN) metabolizing activity of <i>Saccharomyces cerevisiae</i> ChCR11 and INVSc1 expressing <i>CYP3201B1.</i> |
| topic | Biochemistry Evolutionary Biology Ecology Plant Biology Virology Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified subsequently converting aldoxime natural defensive chemicals indicating sequential duplication catalyse aldoxime dehydration div >< p cyanogenic millipedes evolved chamberlinius hualienensis </ hualienensis </ r </ typically lineage related enzymes plant lineages plant kingdoms paralogous genes millipedes demonstrates mechanisms underlying hydroxynitrile lyase hydrogen cyanide giving rise enzymes independently dependent monooxygenase deeper understanding cytochrome p450 complete set coding genes biological characterisation >)- mandelonitrile |