Binding analysis of Cry toxins to gut tissues.

Binding analysis of Cry toxins to gut tissues.

<p>Radiometric analysis of 20–30 lines with a length 5 μm selected from anterior, medium and posterior regions of <i>A. aegypti</i> gut. Fiji software was used to perform a line analysis of fluorescence intensity (IF) of the brush border membrane (BBM) and peritrophic membrane (PM)...

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Détails bibliographiques
Auteur principal: Sabino Pacheco (372889) (author)
Autres auteurs: Marcos Chiñas (11635765) (author), Juan Ulises Gómez (21656025) (author), Ángel Enrique Peláez-Aguilar (21656028) (author), Nathaly Alexandre do Nascimento (21656031) (author), Pablo Emiliano Cantón (8262417) (author), Jorge Sánchez (2204284) (author), Samira López-Molina (10005222) (author), Isabel Gómez (833406) (author), Mario Soberón (322907) (author), Alejandra Bravo (322909) (author)
Publié: 2025
Sujets:
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Physiology
Pharmacology
Evolutionary Biology
Ecology
Cancer
Inorganic Chemistry
Infectious Diseases
Chemical Sciences not elsewhere classified
wild type strains
redundant receptor function
findings provide evidence
div >< p
avoiding resistance development
similar larvicidal activity
bacillus thuringiensis </
apical microvilli membrane
aedes aegypti </
histopathological analysis showed
abc transporters knockout
abc transporter orthologs
insecticidal cry toxins
cry toxins binding
abc ko mutation
cry toxins
insecticidal activity
abc transporters
abc </
vivo </
transporter genes
membrane proteins
binding assays
abc ko
cry receptors
using crispr
transcriptomic regulation
significant reduction
potentially contribute
paralogous genes
insect larvae
induces upregulation
gut tissue
gut epithelium
genome corresponding
fitness cost
cry11ba ).
could mitigate
compensatory mechanism
coleopteran species
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Description
Résumé:<p>Radiometric analysis of 20–30 lines with a length 5 μm selected from anterior, medium and posterior regions of <i>A. aegypti</i> gut. Fiji software was used to perform a line analysis of fluorescence intensity (IF) of the brush border membrane (BBM) and peritrophic membrane (PM).</p> <p>(TIFF)</p>

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