Binding analysis of Cry toxins to gut tissues.

<p>Radiometric analysis of 20–30 lines with a length 5 μm selected from anterior, medium and posterior regions of <i>A. aegypti</i> gut. Fiji software was used to perform a line analysis of fluorescence intensity (IF) of the brush border membrane (BBM) and peritrophic membrane (PM)...

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Bibliographic Details
Main Author:	Sabino Pacheco (372889) (author)
Other Authors:	Marcos Chiñas (11635765) (author), Juan Ulises Gómez (21656025) (author), Ángel Enrique Peláez-Aguilar (21656028) (author), Nathaly Alexandre do Nascimento (21656031) (author), Pablo Emiliano Cantón (8262417) (author), Jorge Sánchez (2204284) (author), Samira López-Molina (10005222) (author), Isabel Gómez (833406) (author), Mario Soberón (322907) (author), Alejandra Bravo (322909) (author)
Published:	2025
Subjects:	Biochemistry Microbiology Cell Biology Genetics Molecular Biology Physiology Pharmacology Evolutionary Biology Ecology Cancer Inorganic Chemistry Infectious Diseases Chemical Sciences not elsewhere classified wild type strains redundant receptor function findings provide evidence div >< p avoiding resistance development similar larvicidal activity bacillus thuringiensis </ apical microvilli membrane aedes aegypti </ histopathological analysis showed abc transporters knockout abc transporter orthologs insecticidal cry toxins cry toxins binding abc ko mutation cry toxins insecticidal activity abc transporters abc </ vivo </ transporter genes membrane proteins binding assays abc ko cry receptors using crispr transcriptomic regulation significant reduction potentially contribute paralogous genes insect larvae induces upregulation gut tissue gut epithelium genome corresponding fitness cost cry11ba ). could mitigate compensatory mechanism coleopteran species
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Binding analysis of Cry toxins to gut tissues.

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