Proteomic analysis reveals key kroteins in exosomes derived from lung fibroblasts of idiopathic pulmonary fibrosis
Primary fibroblasts were isolated from 3 transplanted lung speciments from IPF patients and 3 distal surgical lung specimens from the nodule patients. Exosomes were isolated from the cell culture supernatant using a differential ultracentrifugation method. The enzymatically desalted peptide samples...
محفوظ في:
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| مؤلفون آخرون: | , |
| منشور في: |
2024
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إضافة وسم
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| الملخص: | Primary fibroblasts were isolated from 3 transplanted lung speciments from IPF patients and 3 distal surgical lung specimens from the nodule patients. Exosomes were isolated from the cell culture supernatant using a differential ultracentrifugation method. The enzymatically desalted peptide samples were analyzed by data-independent acquisition mass spectrometry on a QE HF-X mass spectrometer (Thermo Fisher Scientific, USA). |
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