AKB9778 improves SC function in aged mice.

<p>A. Illustration of the topical ocular administration scheme of AKB9778 containing eyedrops or vehicle (twice daily) to 75–85-week-old mice and time points of IOP measurements. B. Confocal microscopy images of cornea whole mounts showing the SC area (green, PECAM-1) of vehicle or AKB9778 tre...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Sarthak Mishra (12369590) (author)
مؤلفون آخرون: Ute Ipe (21377561) (author), Astrid F. Nottebaum (19955940) (author), Kevin G. Peters (21377564) (author), Dietmar Vestweber (92962) (author)
منشور في: 2025
الموضوعات:
الوسوم: إضافة وسم
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الوصف
الملخص:<p>A. Illustration of the topical ocular administration scheme of AKB9778 containing eyedrops or vehicle (twice daily) to 75–85-week-old mice and time points of IOP measurements. B. Confocal microscopy images of cornea whole mounts showing the SC area (green, PECAM-1) of vehicle or AKB9778 treated WT aged mice after 4 weeks of treatment (twice daily). Images shown are maximum intensity projections obtained from Z stacks with a step size of 0.89 µm, captured using a 20X objective. C. Quantification of SC area from vehicle or AKB9778-treated mice using Fiji software. Each dot represents the mean SC area of one mouse averaged over 12-16 20x images. D. IOP values measured before starting (75-85 wk) topical ocular eye drop treatment and at the end (79-89 wk) of the treatment using a rebound tonometer. n = 8 mice per group. Scale Bars: 50µm. p values for SC area were obtained using unpaired two tailed student’s T test and for IOP measurements using two-way ANOVA with Tukey’s multiple comparison; *P ≤ 0.05, **P ≤ 0.01.</p>