ROI Restriction and Visualization in WormSNAP GUI.
<p>(A) ROIs detected by Local Area Thresholding algorithm on the crop of an Overexpressed Synaptic Fluorophore (previously shown in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1013643#pcbi.1013643.g001" target="_blank">Fig 1B</a>)....
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , |
| منشور في: |
2025
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| الملخص: | <p>(A) ROIs detected by Local Area Thresholding algorithm on the crop of an Overexpressed Synaptic Fluorophore (previously shown in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1013643#pcbi.1013643.g001" target="_blank">Fig 1B</a>). (B) Left:Crop Restriction parameters from WormSNAP’s ROI Detection Tab (see <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1013643#pcbi.1013643.s003" target="_blank">S3B Fig</a>), with a y-edge exclusion of 3px (≈0.63µm). Right Top: Magnified view of the blue box (in A) showing no ROIs assigned and illustrating that the bottom-most punctum is part of the gut (outlined in dotted black line). See <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1013643#pcbi.1013643.g001" target="_blank">Fig 1B</a> for the full image showing the gut impinging on the crop outline. Right Bottom: Example of how excluding y-edge pixels can remove a misassigned ROI from the gut granule. Dotted lines indicate the boundaries beyond which ROIs are ignored. The excluded ROI is shown in red. Scale Bar, 5µm. (C) Left: ROI Restriction parameters from WormSNAP’s ROI Detection Tab, set to a minimum area of 5px<sup>2</sup>/0.22µm<sup>2</sup>. Right Top: Magnified view of the red box (in A), showing no ROIs assigned. Right Bottom: Example of how the minimum area setting excludes misassigned ROIs that appear as individual bright pixels due to noise. Excluded ROIs are shown in red. Scale Bar, 5µm. (D-I) Magnified view of WormSNAP’s Results Tab (see <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1013643#pcbi.1013643.s003" target="_blank">S3C Fig</a>) and an example 2-channel crops visualization of 3 crops from the Display Tab, showing: D. Channel 1 (CLA-1::GFP) intensities; E. Channel 1 (CLA-1::GFP) intensities overlaid with ROIs from Channel 1 (CLA-1::GFP) in green; F. Channel 2 (RAB-3::tdTomato) intensities; G. Channel 2 (RAB-3::tdTomato) intensities overlaid with ROIs from Channel 2 (RAB-3::tdTomato) in magenta; H. Channel 2 (RAB-3::tdTomato) intensities overlaid with ROIs from Channel 1 (CLA-1::GFP) in green; I. Channel 1 (CLA-1::GFP) intensities overlaid with Channel 1 (CLA-1::GFP) ROIs in green and Channel 2 (RAB-3::tdTomato) ROIs in magenta. Scale Bars, 5µm.</p> |
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