Engineering of Integral Membrane Metalloenzyme UndB and Designing of a Cell-Free Biocatalytic Platform Enabled Efficient 1‑Alkene Production
The bioproduction of 1-alkenes is of significant global interest due to their potential as green commodity chemicals and next-generation ‘drop-in’ biofuels. Here, we report an engineering strategy to enhance the catalytic activity and substrate specificity of the membrane-bound metalloenzyme UndB, s...
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2025
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