Sensitivity of Δ<i>Fghof1</i> mutant conidia to different DAPI concentrations.
<p>(A) One end of each conidium produced by Δ<i>Fgcyk3</i> mutant usually has conical protrusion (left panel; protrusion is indicated by red arrow). Like Δ<i>Fghof1</i> mutant, some Δ<i>Fgcyk3</i> mutant conidia also appear with end-to-end attachment forming...
Sparad:
| Huvudupphovsman: | |
|---|---|
| Övriga upphovsmän: | , , , , , |
| Publicerad: |
2025
|
| Ämnen: | |
| Taggar: |
Lägg till en tagg
Inga taggar, Lägg till första taggen!
|
| _version_ | 1849927641200590848 |
|---|---|
| author | Yakubu Saddeeq Abubakar (5558399) |
| author2 | Shiqi Ji (20476509) Qiaoli Chen (538344) Huawei Zheng (713784) Zonghua Wang (133934) Wenhui Zheng (133904) Yi Lou (381696) |
| author2_role | author author author author author author |
| author_facet | Yakubu Saddeeq Abubakar (5558399) Shiqi Ji (20476509) Qiaoli Chen (538344) Huawei Zheng (713784) Zonghua Wang (133934) Wenhui Zheng (133904) Yi Lou (381696) |
| author_role | author |
| dc.creator.none.fl_str_mv | Yakubu Saddeeq Abubakar (5558399) Shiqi Ji (20476509) Qiaoli Chen (538344) Huawei Zheng (713784) Zonghua Wang (133934) Wenhui Zheng (133904) Yi Lou (381696) |
| dc.date.none.fl_str_mv | 2025-11-24T18:35:52Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013726.s011 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Sensitivity_of_i_Fghof1_i_mutant_conidia_to_different_DAPI_concentrations_/30697835 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Developmental Biology Infectious Diseases Plant Biology div >< p conserved molecular scaffolds irregular colony edges form protein condensates dynamic protein assembly remaining 20 proteins plant fungal pathogen idrs largely altered proteins exhibited features fusarium graminearum </ graminearum </ fusarium </ protein interaction plant infection mediate protein irregular septation fungal pathogenesis containing proteins wide characterization varied functions systematically identified src homology septal pore previously characterized potential targets plasma membrane nuclear fragmentation nuclear distribution negative regulators mutant strains multifaceted functions hyphal tip head blight functional diversity completely abolished apical collar |
| dc.title.none.fl_str_mv | Sensitivity of Δ<i>Fghof1</i> mutant conidia to different DAPI concentrations. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>(A) One end of each conidium produced by Δ<i>Fgcyk3</i> mutant usually has conical protrusion (left panel; protrusion is indicated by red arrow). Like Δ<i>Fghof1</i> mutant, some Δ<i>Fgcyk3</i> mutant conidia also appear with end-to-end attachment forming a chain of unseparated conidia (middle and right panels). White arrows show the points of attachment of the conidia to one another. The spores were stained with CFW. Bar = 20 µm. (B) At a DAPI concentration of 10 µg/mL, the cytoplasmic contents of Δ<i>Fghof1</i> mutant conidia become coagulated and the mutant dies. Although the mutant survives lower concentrations of DAPI, fragmented nuclei were often observed in the conidia. Bar = 10 µm. (C) Colony growth of PH-1 and Δ<i>Fghof1</i> mutant in the presence of 0.005% methyl methane sulfonate (MMS). Statistical analysis was processed by one-way ANOVA for multiple comparisons using GRAPHPAD PRISM 9 (Data represent means ± SD, Student’s t-test; *, P < 0.05). (D) Analysis of growth inhibition rate of Δ<i>Fghof1</i> mutant relative to PH-1 on CM media containing 0.005% MMS.</p> <p>(TIF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_ec81fe025e6620b116d9de0ce70d8530 |
| identifier_str_mv | 10.1371/journal.ppat.1013726.s011 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697835 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Sensitivity of Δ<i>Fghof1</i> mutant conidia to different DAPI concentrations.Yakubu Saddeeq Abubakar (5558399)Shiqi Ji (20476509)Qiaoli Chen (538344)Huawei Zheng (713784)Zonghua Wang (133934)Wenhui Zheng (133904)Yi Lou (381696)BiophysicsBiochemistryMedicineMicrobiologyCell BiologyGeneticsDevelopmental BiologyInfectious DiseasesPlant Biologydiv >< pconserved molecular scaffoldsirregular colony edgesform protein condensatesdynamic protein assemblyremaining 20 proteinsplant fungal pathogenidrs largely alteredproteins exhibited featuresfusarium graminearum </graminearum </fusarium </protein interactionplant infectionmediate proteinirregular septationfungal pathogenesiscontaining proteinswide characterizationvaried functionssystematically identifiedsrc homologyseptal porepreviously characterizedpotential targetsplasma membranenuclear fragmentationnuclear distributionnegative regulatorsmutant strainsmultifaceted functionshyphal tiphead blightfunctional diversitycompletely abolishedapical collar<p>(A) One end of each conidium produced by Δ<i>Fgcyk3</i> mutant usually has conical protrusion (left panel; protrusion is indicated by red arrow). Like Δ<i>Fghof1</i> mutant, some Δ<i>Fgcyk3</i> mutant conidia also appear with end-to-end attachment forming a chain of unseparated conidia (middle and right panels). White arrows show the points of attachment of the conidia to one another. The spores were stained with CFW. Bar = 20 µm. (B) At a DAPI concentration of 10 µg/mL, the cytoplasmic contents of Δ<i>Fghof1</i> mutant conidia become coagulated and the mutant dies. Although the mutant survives lower concentrations of DAPI, fragmented nuclei were often observed in the conidia. Bar = 10 µm. (C) Colony growth of PH-1 and Δ<i>Fghof1</i> mutant in the presence of 0.005% methyl methane sulfonate (MMS). Statistical analysis was processed by one-way ANOVA for multiple comparisons using GRAPHPAD PRISM 9 (Data represent means ± SD, Student’s t-test; *, P < 0.05). (D) Analysis of growth inhibition rate of Δ<i>Fghof1</i> mutant relative to PH-1 on CM media containing 0.005% MMS.</p> <p>(TIF)</p>2025-11-24T18:35:52ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013726.s011https://figshare.com/articles/figure/Sensitivity_of_i_Fghof1_i_mutant_conidia_to_different_DAPI_concentrations_/30697835CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306978352025-11-24T18:35:52Z |
| spellingShingle | Sensitivity of Δ<i>Fghof1</i> mutant conidia to different DAPI concentrations. Yakubu Saddeeq Abubakar (5558399) Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Developmental Biology Infectious Diseases Plant Biology div >< p conserved molecular scaffolds irregular colony edges form protein condensates dynamic protein assembly remaining 20 proteins plant fungal pathogen idrs largely altered proteins exhibited features fusarium graminearum </ graminearum </ fusarium </ protein interaction plant infection mediate protein irregular septation fungal pathogenesis containing proteins wide characterization varied functions systematically identified src homology septal pore previously characterized potential targets plasma membrane nuclear fragmentation nuclear distribution negative regulators mutant strains multifaceted functions hyphal tip head blight functional diversity completely abolished apical collar |
| status_str | publishedVersion |
| title | Sensitivity of Δ<i>Fghof1</i> mutant conidia to different DAPI concentrations. |
| title_full | Sensitivity of Δ<i>Fghof1</i> mutant conidia to different DAPI concentrations. |
| title_fullStr | Sensitivity of Δ<i>Fghof1</i> mutant conidia to different DAPI concentrations. |
| title_full_unstemmed | Sensitivity of Δ<i>Fghof1</i> mutant conidia to different DAPI concentrations. |
| title_short | Sensitivity of Δ<i>Fghof1</i> mutant conidia to different DAPI concentrations. |
| title_sort | Sensitivity of Δ<i>Fghof1</i> mutant conidia to different DAPI concentrations. |
| topic | Biophysics Biochemistry Medicine Microbiology Cell Biology Genetics Developmental Biology Infectious Diseases Plant Biology div >< p conserved molecular scaffolds irregular colony edges form protein condensates dynamic protein assembly remaining 20 proteins plant fungal pathogen idrs largely altered proteins exhibited features fusarium graminearum </ graminearum </ fusarium </ protein interaction plant infection mediate protein irregular septation fungal pathogenesis containing proteins wide characterization varied functions systematically identified src homology septal pore previously characterized potential targets plasma membrane nuclear fragmentation nuclear distribution negative regulators mutant strains multifaceted functions hyphal tip head blight functional diversity completely abolished apical collar |