Figure 6 from T Cells Promote Metastasis by Regulating Extracellular Matrix Remodeling following Chemotherapy
<p>LOX-expressing CD8<sup>+</sup> T cells promote pulmonary ECM remodeling in response to chemotherapy. Tumor-free, 8- to 10-week-old BALB/c mice (<i>n</i> = 5 mice/group) were treated with PTX or vehicle control. After 72 hours, lungs and spleens were removed. <b>...
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2025
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| Sumario: | <p>LOX-expressing CD8<sup>+</sup> T cells promote pulmonary ECM remodeling in response to chemotherapy. Tumor-free, 8- to 10-week-old BALB/c mice (<i>n</i> = 5 mice/group) were treated with PTX or vehicle control. After 72 hours, lungs and spleens were removed. <b>A–C,</b> LOX intensity presented by histogram and bar graph was assessed by flow cytometry (left), and LOX mRNA levels were assessed by RT-qPCR (right) in CD8<sup>+</sup> T cells (<b>A</b>), CD4<sup>+</sup> T cells (<b>B</b>), and B cells (<b>C</b>) in lung tissue. Lymphocyte percentages are shown for the flow cytometry analysis. <b>D–G,</b> CD8<sup>+</sup> T cells, isolated from the spleens of control- and PTX-treated BALB/c mice, were adoptively transferred by injection through the tail vein into naïve SCID mice (2.5 × 10<sup>6</sup> cells/mouse; <i>n</i> = 6–9 mice/group). Control SCID mice were not injected with T cells (naïve). After 72 hours, lungs were removed and analyzed as follows: <b>D,</b> Collagen and elastin were detected by Sirius red staining. Representative images are shown in two magnifications. Scale bar, 200 μm. Arrows, positive staining. <b>E,</b> Quantification of the percentage of positive pixels per total lung area (<i>n</i> > 4 fields/lung) is shown. <b>F,</b> LOX was detected by immunostaining. Representative images of LOX staining (red) and nuclear counterstaining (blue) are shown. Scale bar, 50 μm. <b>G,</b> A quantification of positive red pixels representing LOX expression per field (<i>n</i> > 4 fields/lung) is shown. Statistical significance was assessed by one-way ANOVA followed by Tukey posttest when more than two groups were analyzed or unpaired two-tailed <i>t</i> test when only two groups were analyzed. Significant <i>P</i> values are shown as *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001 from control or otherwise indicated in the figure.</p> |
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