cell cycle orignal date
<p dir="ltr">Following trypsinization, glioma cells were collected and adjusted to a density of 1×10⁶ cells per sample in a 15 mL conical centrifuge tube. The cells were washed twice with pre-cooled PBS and fixed in 70% ethanol overnight at 4°C. Subsequently, the fixed cells were sta...
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2025
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| _version_ | 1849927635216367616 |
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| author | junhong dong (22092350) |
| author_facet | junhong dong (22092350) |
| author_role | author |
| dc.creator.none.fl_str_mv | junhong dong (22092350) |
| dc.date.none.fl_str_mv | 2025-11-25T08:10:51Z |
| dc.identifier.none.fl_str_mv | 10.6084/m9.figshare.29950406.v4 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/cell_cycle_zipcell_cycle_/29950406 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Cell development, proliferation and death cell cycle arrest genes ASIC2 |
| dc.title.none.fl_str_mv | cell cycle orignal date |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <p dir="ltr">Following trypsinization, glioma cells were collected and adjusted to a density of 1×10⁶ cells per sample in a 15 mL conical centrifuge tube. The cells were washed twice with pre-cooled PBS and fixed in 70% ethanol overnight at 4°C. Subsequently, the fixed cells were stained with propidium iodide according to the manufacturer's instructions of the cell cycle detection kit (Beyotime, Shanghai, China). DNA content analysis was then performed using a flow cytometer (BD Accuri C6 Plus), and the resulting data were analyzed to determine the cell cycle distribution.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_f0597b9f3ca840b5620dd3f969569bba |
| identifier_str_mv | 10.6084/m9.figshare.29950406.v4 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/29950406 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | cell cycle orignal datejunhong dong (22092350)Cell development, proliferation and deathcell cycle arrest genesASIC2<p dir="ltr">Following trypsinization, glioma cells were collected and adjusted to a density of 1×10⁶ cells per sample in a 15 mL conical centrifuge tube. The cells were washed twice with pre-cooled PBS and fixed in 70% ethanol overnight at 4°C. Subsequently, the fixed cells were stained with propidium iodide according to the manufacturer's instructions of the cell cycle detection kit (Beyotime, Shanghai, China). DNA content analysis was then performed using a flow cytometer (BD Accuri C6 Plus), and the resulting data were analyzed to determine the cell cycle distribution.</p>2025-11-25T08:10:51ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.6084/m9.figshare.29950406.v4https://figshare.com/articles/dataset/cell_cycle_zipcell_cycle_/29950406CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/299504062025-11-25T08:10:51Z |
| spellingShingle | cell cycle orignal date junhong dong (22092350) Cell development, proliferation and death cell cycle arrest genes ASIC2 |
| status_str | publishedVersion |
| title | cell cycle orignal date |
| title_full | cell cycle orignal date |
| title_fullStr | cell cycle orignal date |
| title_full_unstemmed | cell cycle orignal date |
| title_short | cell cycle orignal date |
| title_sort | cell cycle orignal date |
| topic | Cell development, proliferation and death cell cycle arrest genes ASIC2 |