Additional file 9 of Common cell lysis procedures distort ribosome profiling analyses of gene expression
Additional file 9: Fig. S3. Analysis of the effects of omission of centrifugation on riboseq and RNAseq reads aligned to MACF1 genomic locus A) Genomic alignments of riboseq with centrifugation (blue) and without centrifugation (red), related to Fig 2B, and alignments of RNAseq from cell supernatant...
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2025
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| Summary: | Additional file 9: Fig. S3. Analysis of the effects of omission of centrifugation on riboseq and RNAseq reads aligned to MACF1 genomic locus A) Genomic alignments of riboseq with centrifugation (blue) and without centrifugation (red), related to Fig 2B, and alignments of RNAseq from cell supernatant (blue) and cell pellet (red), related to Fig. 2B, and alignments of RNAseq from cell supernatantand cell pellet, related to Fig. 2A. Region where read density increases in centrifugated and supernatant samples is highlighted in blue. First exon of shorter mRNA isoform which likely corresponds to NM_001397473.1 contain multiple translated uORFs which are highlighted by violet arrows. B) RNAseq reads aligned to longer mRNA ENST00000361689 of supernatant and pellet samples related to Fig. 2A. |
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