Tissue-specific analysis of RVA RNA levels in extraintestinal tissues.
<p>Six-day-old germ-free pigs were orally inoculated with 1 × 106 FFU of rotavirus. At the post inoculation times indicated, animals were euthanized and tissues (lungs, salivary glands, liver, blood and spleen) were collected. (A) Levels of RVA RNA in extraintestinal tissues across dpi 1–4. (B...
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2025
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| _version_ | 1849927626899062784 |
|---|---|
| author | Sergei A. Raev (15350287) |
| author2 | Maryssa K. Kick (17183901) Maria Chellis (22683628) Linda J. Saif (6626666) Talita P. Resende (3916268) Anastasia N. Vlasova (6626663) |
| author2_role | author author author author author |
| author_facet | Sergei A. Raev (15350287) Maryssa K. Kick (17183901) Maria Chellis (22683628) Linda J. Saif (6626666) Talita P. Resende (3916268) Anastasia N. Vlasova (6626663) |
| author_role | author |
| dc.creator.none.fl_str_mv | Sergei A. Raev (15350287) Maryssa K. Kick (17183901) Maria Chellis (22683628) Linda J. Saif (6626666) Talita P. Resende (3916268) Anastasia N. Vlasova (6626663) |
| dc.date.none.fl_str_mv | 2025-11-25T18:38:39Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.ppat.1013723.s002 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Tissue-specific_analysis_of_RVA_RNA_levels_in_extraintestinal_tissues_/30714670 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Microbiology Ecology Infectious Diseases Space Science Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified increased sialic acid host characteristics regulate first experimental evidence findings may provide current global dominance rva rna detection multiple extraintestinal organs div >< p rva strains replicated related rva strains porcine osu g5p facilitating viral dissemination efficient extraintestinal spread rva receptor diversity rva extraintestinal spread examined extraintestinal tissues gut </ p extraintestinal tissues extraintestinal spread extraintestinal tissue extraintestinal sites also examined vivo </ vitro </ different tissues well understood respiratory lesions remarkably robust potential role pigs infected moderate titers microscopic lesions intestinal tissue inoculated germ highest titers highest frequency higher rate free pigs determine whether better understand |
| dc.title.none.fl_str_mv | Tissue-specific analysis of RVA RNA levels in extraintestinal tissues. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p>Six-day-old germ-free pigs were orally inoculated with 1 × 106 FFU of rotavirus. At the post inoculation times indicated, animals were euthanized and tissues (lungs, salivary glands, liver, blood and spleen) were collected. (A) Levels of RVA RNA in extraintestinal tissues across dpi 1–4. (B) RT-qPCR RVA RNA detection frequency in extraintestinal tissues across dpi 1–4. (C) ISH RT-qPCR RVA RNA detection frequency in extraintestinal tissues across dpi 1–4 and (D) at time points when significant differences were observed. Statistical analysis for RVA RNA detection frequency was performed by using Fisher’s exact test. Significant differences (*p < 0.05) are indicated.</p> <p>(TIF)</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_f3c47851a46dbd43618bef3f119562d4 |
| identifier_str_mv | 10.1371/journal.ppat.1013723.s002 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30714670 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Tissue-specific analysis of RVA RNA levels in extraintestinal tissues.Sergei A. Raev (15350287)Maryssa K. Kick (17183901)Maria Chellis (22683628)Linda J. Saif (6626666)Talita P. Resende (3916268)Anastasia N. Vlasova (6626663)BiophysicsBiochemistryMicrobiologyEcologyInfectious DiseasesSpace ScienceEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedincreased sialic acidhost characteristics regulatefirst experimental evidencefindings may providecurrent global dominancerva rna detectionmultiple extraintestinal organsdiv >< prva strains replicatedrelated rva strainsporcine osu g5pfacilitating viral disseminationefficient extraintestinal spreadrva receptor diversityrva extraintestinal spreadexamined extraintestinal tissuesgut </ pextraintestinal tissuesextraintestinal spreadextraintestinal tissueextraintestinal sitesalso examinedvivo </vitro </different tissueswell understoodrespiratory lesionsremarkably robustpotential rolepigs infectedmoderate titersmicroscopic lesionsintestinal tissueinoculated germhighest titershighest frequencyhigher ratefree pigsdetermine whetherbetter understand<p>Six-day-old germ-free pigs were orally inoculated with 1 × 106 FFU of rotavirus. At the post inoculation times indicated, animals were euthanized and tissues (lungs, salivary glands, liver, blood and spleen) were collected. (A) Levels of RVA RNA in extraintestinal tissues across dpi 1–4. (B) RT-qPCR RVA RNA detection frequency in extraintestinal tissues across dpi 1–4. (C) ISH RT-qPCR RVA RNA detection frequency in extraintestinal tissues across dpi 1–4 and (D) at time points when significant differences were observed. Statistical analysis for RVA RNA detection frequency was performed by using Fisher’s exact test. Significant differences (*p < 0.05) are indicated.</p> <p>(TIF)</p>2025-11-25T18:38:39ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.ppat.1013723.s002https://figshare.com/articles/figure/Tissue-specific_analysis_of_RVA_RNA_levels_in_extraintestinal_tissues_/30714670CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307146702025-11-25T18:38:39Z |
| spellingShingle | Tissue-specific analysis of RVA RNA levels in extraintestinal tissues. Sergei A. Raev (15350287) Biophysics Biochemistry Microbiology Ecology Infectious Diseases Space Science Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified increased sialic acid host characteristics regulate first experimental evidence findings may provide current global dominance rva rna detection multiple extraintestinal organs div >< p rva strains replicated related rva strains porcine osu g5p facilitating viral dissemination efficient extraintestinal spread rva receptor diversity rva extraintestinal spread examined extraintestinal tissues gut </ p extraintestinal tissues extraintestinal spread extraintestinal tissue extraintestinal sites also examined vivo </ vitro </ different tissues well understood respiratory lesions remarkably robust potential role pigs infected moderate titers microscopic lesions intestinal tissue inoculated germ highest titers highest frequency higher rate free pigs determine whether better understand |
| status_str | publishedVersion |
| title | Tissue-specific analysis of RVA RNA levels in extraintestinal tissues. |
| title_full | Tissue-specific analysis of RVA RNA levels in extraintestinal tissues. |
| title_fullStr | Tissue-specific analysis of RVA RNA levels in extraintestinal tissues. |
| title_full_unstemmed | Tissue-specific analysis of RVA RNA levels in extraintestinal tissues. |
| title_short | Tissue-specific analysis of RVA RNA levels in extraintestinal tissues. |
| title_sort | Tissue-specific analysis of RVA RNA levels in extraintestinal tissues. |
| topic | Biophysics Biochemistry Microbiology Ecology Infectious Diseases Space Science Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified increased sialic acid host characteristics regulate first experimental evidence findings may provide current global dominance rva rna detection multiple extraintestinal organs div >< p rva strains replicated related rva strains porcine osu g5p facilitating viral dissemination efficient extraintestinal spread rva receptor diversity rva extraintestinal spread examined extraintestinal tissues gut </ p extraintestinal tissues extraintestinal spread extraintestinal tissue extraintestinal sites also examined vivo </ vitro </ different tissues well understood respiratory lesions remarkably robust potential role pigs infected moderate titers microscopic lesions intestinal tissue inoculated germ highest titers highest frequency higher rate free pigs determine whether better understand |