Analysis of rCHIKV-E1 Protein Expression and Purification.

(A) Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showing purity of His-tagged E1 protein of CHIKV. Marker, prestained protein ladder; Lane 1, Without IPTG induced crude E. coli lysates; Lane 2, IPTG-induced E. coli lysate expres...

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Номзүйн дэлгэрэнгүй
Үндсэн зохиолч:	Kun Wen (790021) (author)
Бусад зохиолчид:	Zhihong Zhou (353166) (author), Peipei Xu (391804) (author), Biao Di (284801) (author), Jialing Song (15283367) (author), Cuilian Yang (22784181) (author), Lili Zhan (22784184) (author), Chongquan Zhao (22784187) (author), Mei Xu (188070) (author), Jianpiao Cai (9857938) (author), Shuofeng Yuan (2827973) (author), Kelvin Kai-Wang To (9144227) (author), Wenxi Feng (15304580) (author), Jasper Fuk-Woo Chan (8085932) (author), Hongwei Zhou (93199) (author), Yue Chen (73753) (author), Xixia Ding (567730) (author)
Хэвлэсэн:	2025
Нөхцлүүд:	Medicine Microbiology Biotechnology Immunology Cancer Science Policy Mental Health Infectious Diseases Plant Biology Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified escherichia coli </ 3 – 99 3 – 97 including dengue virus div >< p chikv e1 protein including guangdong e1 protein chikungunya virus viral entry therapeutic target serum samples reference standard promising diagnostic previously non monoclonal antibodies major human infected patients infected controls increasingly reported enzyme immunoassay endemic areas eia ). e1 antigen denv ). chikv infection chikungunya fever assay achieved accurate diagnosis 93 healthy 84 chikv 5 ).
Шошгууд:	Шошго нэмэх Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!

Тодорхойлолт
Тойм:	<p><b>(A)</b> Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showing purity of His-tagged E1 protein of CHIKV. Marker, prestained protein ladder; Lane 1, Without IPTG induced crude <i>E. coli</i> lysates; Lane 2, IPTG-induced E. coli lysate expressing pET-21b (+) with CHIKV-E1 cDNA; Lane 3, purified rCHIKV-E1 protein; Lane 4, refolded rCHIKV-E1 protein. <b>(B)</b> Western blot analysis of refolded rCHIKV-E1 to confirm molecular weight and specificity using monoclonal antibodies (mAbs) produced in our laboratory. Lane 1: mouse anti-His mAb (molecular weight confirmation); Lanes 2–3: mouse anti-DENV-ED3 mAbs (5A1A3, 5F29A6, negative controls); Lane 4: mouse anti-DENV-NS1 mAb (5A11A5, negative control); Lanes 5–6: mouse anti-WNV-NS1 mAbs (WNVNS1-M66, WNVNS1-M2, negative controls).</p>

Analysis of rCHIKV-E1 Protein Expression and Purification.

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