Analysis of rCHIKV-E1 Protein Expression and Purification.

(A) Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showing purity of His-tagged E1 protein of CHIKV. Marker, prestained protein ladder; Lane 1, Without IPTG induced crude E. coli lysates; Lane 2, IPTG-induced E. coli lysate expres...

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第一著者:	Kun Wen (790021) (author)
その他の著者:	Zhihong Zhou (353166) (author), Peipei Xu (391804) (author), Biao Di (284801) (author), Jialing Song (15283367) (author), Cuilian Yang (22784181) (author), Lili Zhan (22784184) (author), Chongquan Zhao (22784187) (author), Mei Xu (188070) (author), Jianpiao Cai (9857938) (author), Shuofeng Yuan (2827973) (author), Kelvin Kai-Wang To (9144227) (author), Wenxi Feng (15304580) (author), Jasper Fuk-Woo Chan (8085932) (author), Hongwei Zhou (93199) (author), Yue Chen (73753) (author), Xixia Ding (567730) (author)
出版事項:	2025
主題:	Medicine Microbiology Biotechnology Immunology Cancer Science Policy Mental Health Infectious Diseases Plant Biology Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified escherichia coli </ 3 – 99 3 – 97 including dengue virus div >< p chikv e1 protein including guangdong e1 protein chikungunya virus viral entry therapeutic target serum samples reference standard promising diagnostic previously non monoclonal antibodies major human infected patients infected controls increasingly reported enzyme immunoassay endemic areas eia ). e1 antigen denv ). chikv infection chikungunya fever assay achieved accurate diagnosis 93 healthy 84 chikv 5 ).
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_version_	1851480487591149568
author	Kun Wen (790021)
author2	Zhihong Zhou (353166) Peipei Xu (391804) Biao Di (284801) Jialing Song (15283367) Cuilian Yang (22784181) Lili Zhan (22784184) Chongquan Zhao (22784187) Mei Xu (188070) Jianpiao Cai (9857938) Shuofeng Yuan (2827973) Kelvin Kai-Wang To (9144227) Wenxi Feng (15304580) Jasper Fuk-Woo Chan (8085932) Hongwei Zhou (93199) Yue Chen (73753) Xixia Ding (567730)
author2_role	author author author author author author author author author author author author author author author author
author_facet	Kun Wen (790021) Zhihong Zhou (353166) Peipei Xu (391804) Biao Di (284801) Jialing Song (15283367) Cuilian Yang (22784181) Lili Zhan (22784184) Chongquan Zhao (22784187) Mei Xu (188070) Jianpiao Cai (9857938) Shuofeng Yuan (2827973) Kelvin Kai-Wang To (9144227) Wenxi Feng (15304580) Jasper Fuk-Woo Chan (8085932) Hongwei Zhou (93199) Yue Chen (73753) Xixia Ding (567730)
author_role	author
dc.creator.none.fl_str_mv	Kun Wen (790021) Zhihong Zhou (353166) Peipei Xu (391804) Biao Di (284801) Jialing Song (15283367) Cuilian Yang (22784181) Lili Zhan (22784184) Chongquan Zhao (22784187) Mei Xu (188070) Jianpiao Cai (9857938) Shuofeng Yuan (2827973) Kelvin Kai-Wang To (9144227) Wenxi Feng (15304580) Jasper Fuk-Woo Chan (8085932) Hongwei Zhou (93199) Yue Chen (73753) Xixia Ding (567730)
dc.date.none.fl_str_mv	2025-12-04T19:20:24Z
dc.identifier.none.fl_str_mv	10.1371/journal.pntd.0013794.g001
dc.relation.none.fl_str_mv	https://figshare.com/articles/figure/Analysis_of_rCHIKV-E1_Protein_Expression_and_Purification_/30794847
dc.rights.none.fl_str_mv	CC BY 4.0 info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv	Medicine Microbiology Biotechnology Immunology Cancer Science Policy Mental Health Infectious Diseases Plant Biology Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified escherichia coli </ 3 – 99 3 – 97 including dengue virus div >< p chikv e1 protein including guangdong e1 protein chikungunya virus viral entry therapeutic target serum samples reference standard promising diagnostic previously non monoclonal antibodies major human infected patients infected controls increasingly reported enzyme immunoassay endemic areas eia ). e1 antigen denv ). chikv infection chikungunya fever assay achieved accurate diagnosis 93 healthy 84 chikv 5 ).
dc.title.none.fl_str_mv	Analysis of rCHIKV-E1 Protein Expression and Purification.
dc.type.none.fl_str_mv	Image Figure info:eu-repo/semantics/publishedVersion image
description	<p><b>(A)</b> Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showing purity of His-tagged E1 protein of CHIKV. Marker, prestained protein ladder; Lane 1, Without IPTG induced crude <i>E. coli</i> lysates; Lane 2, IPTG-induced E. coli lysate expressing pET-21b (+) with CHIKV-E1 cDNA; Lane 3, purified rCHIKV-E1 protein; Lane 4, refolded rCHIKV-E1 protein. <b>(B)</b> Western blot analysis of refolded rCHIKV-E1 to confirm molecular weight and specificity using monoclonal antibodies (mAbs) produced in our laboratory. Lane 1: mouse anti-His mAb (molecular weight confirmation); Lanes 2–3: mouse anti-DENV-ED3 mAbs (5A1A3, 5F29A6, negative controls); Lane 4: mouse anti-DENV-NS1 mAb (5A11A5, negative control); Lanes 5–6: mouse anti-WNV-NS1 mAbs (WNVNS1-M66, WNVNS1-M2, negative controls).</p>
eu_rights_str_mv	openAccess
id	Manara_f558ea76fce2c5c87b8e02daf6c402f5
identifier_str_mv	10.1371/journal.pntd.0013794.g001
network_acronym_str	Manara
network_name_str	ManaraRepo
oai_identifier_str	oai:figshare.com:article/30794847
publishDate	2025
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv	CC BY 4.0
spelling	Analysis of rCHIKV-E1 Protein Expression and Purification.Kun Wen (790021)Zhihong Zhou (353166)Peipei Xu (391804)Biao Di (284801)Jialing Song (15283367)Cuilian Yang (22784181)Lili Zhan (22784184)Chongquan Zhao (22784187)Mei Xu (188070)Jianpiao Cai (9857938)Shuofeng Yuan (2827973)Kelvin Kai-Wang To (9144227)Wenxi Feng (15304580)Jasper Fuk-Woo Chan (8085932)Hongwei Zhou (93199)Yue Chen (73753)Xixia Ding (567730)MedicineMicrobiologyBiotechnologyImmunologyCancerScience PolicyMental HealthInfectious DiseasesPlant BiologyVirologyEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedescherichia coli </3 – 993 – 97including dengue virusdiv >< pchikv e1 proteinincluding guangdonge1 proteinchikungunya virusviral entrytherapeutic targetserum samplesreference standardpromising diagnosticpreviously nonmonoclonal antibodiesmajor humaninfected patientsinfected controlsincreasingly reportedenzyme immunoassayendemic areaseia ).e1 antigendenv ).chikv infectionchikungunya feverassay achievedaccurate diagnosis93 healthy84 chikv5 ).<p><b>(A)</b> Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showing purity of His-tagged E1 protein of CHIKV. Marker, prestained protein ladder; Lane 1, Without IPTG induced crude <i>E. coli</i> lysates; Lane 2, IPTG-induced E. coli lysate expressing pET-21b (+) with CHIKV-E1 cDNA; Lane 3, purified rCHIKV-E1 protein; Lane 4, refolded rCHIKV-E1 protein. <b>(B)</b> Western blot analysis of refolded rCHIKV-E1 to confirm molecular weight and specificity using monoclonal antibodies (mAbs) produced in our laboratory. Lane 1: mouse anti-His mAb (molecular weight confirmation); Lanes 2–3: mouse anti-DENV-ED3 mAbs (5A1A3, 5F29A6, negative controls); Lane 4: mouse anti-DENV-NS1 mAb (5A11A5, negative control); Lanes 5–6: mouse anti-WNV-NS1 mAbs (WNVNS1-M66, WNVNS1-M2, negative controls).</p>2025-12-04T19:20:24ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pntd.0013794.g001https://figshare.com/articles/figure/Analysis_of_rCHIKV-E1_Protein_Expression_and_Purification_/30794847CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307948472025-12-04T19:20:24Z
spellingShingle	Analysis of rCHIKV-E1 Protein Expression and Purification. Kun Wen (790021) Medicine Microbiology Biotechnology Immunology Cancer Science Policy Mental Health Infectious Diseases Plant Biology Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified escherichia coli </ 3 – 99 3 – 97 including dengue virus div >< p chikv e1 protein including guangdong e1 protein chikungunya virus viral entry therapeutic target serum samples reference standard promising diagnostic previously non monoclonal antibodies major human infected patients infected controls increasingly reported enzyme immunoassay endemic areas eia ). e1 antigen denv ). chikv infection chikungunya fever assay achieved accurate diagnosis 93 healthy 84 chikv 5 ).
status_str	publishedVersion
title	Analysis of rCHIKV-E1 Protein Expression and Purification.
title_full	Analysis of rCHIKV-E1 Protein Expression and Purification.
title_fullStr	Analysis of rCHIKV-E1 Protein Expression and Purification.
title_full_unstemmed	Analysis of rCHIKV-E1 Protein Expression and Purification.
title_short	Analysis of rCHIKV-E1 Protein Expression and Purification.
title_sort	Analysis of rCHIKV-E1 Protein Expression and Purification.
topic	Medicine Microbiology Biotechnology Immunology Cancer Science Policy Mental Health Infectious Diseases Plant Biology Virology Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified escherichia coli </ 3 – 99 3 – 97 including dengue virus div >< p chikv e1 protein including guangdong e1 protein chikungunya virus viral entry therapeutic target serum samples reference standard promising diagnostic previously non monoclonal antibodies major human infected patients infected controls increasingly reported enzyme immunoassay endemic areas eia ). e1 antigen denv ). chikv infection chikungunya fever assay achieved accurate diagnosis 93 healthy 84 chikv 5 ).

Analysis of rCHIKV-E1 Protein Expression and Purification.

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