Genetic Screen Identifies YajC as an LdrA-Neutralizing Factor.
<p><b>(A)</b> Schematic map of the <i>E. coli</i> chromosomal region containing <i>yajC</i>, identified in four independent complementing clones from the genomic library. Arrows indicate the orientation and extent of the genes in this region. The minimal ove...
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2025
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| _version_ | 1849927642744094720 |
|---|---|
| author | Yuto Nakamura (1493086) |
| author2 | Yoshihiro Yamaguchi (329528) |
| author2_role | author |
| author_facet | Yuto Nakamura (1493086) Yoshihiro Yamaguchi (329528) |
| author_role | author |
| dc.creator.none.fl_str_mv | Yuto Nakamura (1493086) Yoshihiro Yamaguchi (329528) |
| dc.date.none.fl_str_mv | 2025-11-24T18:28:27Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0336059.g002 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/figure/Genetic_Screen_Identifies_YajC_as_an_LdrA-Neutralizing_Factor_/30697088 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biochemistry Microbiology Cell Biology Genetics Molecular Biology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified transient growth arrest escherichia coli </ div >< p cells subsequently recover ldr toxin family small membrane protein forming toxin ldra forming toxin damaging toxin accessory protein ldra toxicity ldra results ldra requires including ldra >, ldra suppressive activity results indicate present study novel function molecular basis membrane localization known role key factor homolog ldrd genetic screen findings reveal cytoplasmic domains |
| dc.title.none.fl_str_mv | Genetic Screen Identifies YajC as an LdrA-Neutralizing Factor. |
| dc.type.none.fl_str_mv | Image Figure info:eu-repo/semantics/publishedVersion image |
| description | <p><b>(A)</b> Schematic map of the <i>E. coli</i> chromosomal region containing <i>yajC</i>, identified in four independent complementing clones from the genomic library. Arrows indicate the orientation and extent of the genes in this region. The minimal overlapping region in the complementing clones is indicated. <b>(B)</b> Deletion of <i>yajC</i> abolishes LdrA resistance. <i>E. coli</i> MG1655 cells carrying pTC-<i>ldrA</i> were co-transformed with either empty pUC19 vector, the complementing library fragment pUC19−7, or pUC19−7 carrying a deletion of the <i>yajC</i> open reading frame (pUC19-7-Δ<i>yajC</i>). Cells were streaked on LB agar plates with or without Tc (0.25 µg/ml) and incubated at 37°C for 16 h.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara_f5ff866520e1da625eaac68edb43487b |
| identifier_str_mv | 10.1371/journal.pone.0336059.g002 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30697088 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Genetic Screen Identifies YajC as an LdrA-Neutralizing Factor.Yuto Nakamura (1493086)Yoshihiro Yamaguchi (329528)BiochemistryMicrobiologyCell BiologyGeneticsMolecular BiologyInfectious DiseasesEnvironmental Sciences not elsewhere classifiedBiological Sciences not elsewhere classifiedtransient growth arrestescherichia coli </div >< pcells subsequently recoverldr toxin familysmall membrane proteinforming toxin ldraforming toxindamaging toxinaccessory proteinldra toxicityldra resultsldra requiresincluding ldra>, ldrasuppressive activityresults indicatepresent studynovel functionmolecular basismembrane localizationknown rolekey factorhomolog ldrdgenetic screenfindings revealcytoplasmic domains<p><b>(A)</b> Schematic map of the <i>E. coli</i> chromosomal region containing <i>yajC</i>, identified in four independent complementing clones from the genomic library. Arrows indicate the orientation and extent of the genes in this region. The minimal overlapping region in the complementing clones is indicated. <b>(B)</b> Deletion of <i>yajC</i> abolishes LdrA resistance. <i>E. coli</i> MG1655 cells carrying pTC-<i>ldrA</i> were co-transformed with either empty pUC19 vector, the complementing library fragment pUC19−7, or pUC19−7 carrying a deletion of the <i>yajC</i> open reading frame (pUC19-7-Δ<i>yajC</i>). Cells were streaked on LB agar plates with or without Tc (0.25 µg/ml) and incubated at 37°C for 16 h.</p>2025-11-24T18:28:27ZImageFigureinfo:eu-repo/semantics/publishedVersionimage10.1371/journal.pone.0336059.g002https://figshare.com/articles/figure/Genetic_Screen_Identifies_YajC_as_an_LdrA-Neutralizing_Factor_/30697088CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/306970882025-11-24T18:28:27Z |
| spellingShingle | Genetic Screen Identifies YajC as an LdrA-Neutralizing Factor. Yuto Nakamura (1493086) Biochemistry Microbiology Cell Biology Genetics Molecular Biology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified transient growth arrest escherichia coli </ div >< p cells subsequently recover ldr toxin family small membrane protein forming toxin ldra forming toxin damaging toxin accessory protein ldra toxicity ldra results ldra requires including ldra >, ldra suppressive activity results indicate present study novel function molecular basis membrane localization known role key factor homolog ldrd genetic screen findings reveal cytoplasmic domains |
| status_str | publishedVersion |
| title | Genetic Screen Identifies YajC as an LdrA-Neutralizing Factor. |
| title_full | Genetic Screen Identifies YajC as an LdrA-Neutralizing Factor. |
| title_fullStr | Genetic Screen Identifies YajC as an LdrA-Neutralizing Factor. |
| title_full_unstemmed | Genetic Screen Identifies YajC as an LdrA-Neutralizing Factor. |
| title_short | Genetic Screen Identifies YajC as an LdrA-Neutralizing Factor. |
| title_sort | Genetic Screen Identifies YajC as an LdrA-Neutralizing Factor. |
| topic | Biochemistry Microbiology Cell Biology Genetics Molecular Biology Infectious Diseases Environmental Sciences not elsewhere classified Biological Sciences not elsewhere classified transient growth arrest escherichia coli </ div >< p cells subsequently recover ldr toxin family small membrane protein forming toxin ldra forming toxin damaging toxin accessory protein ldra toxicity ldra results ldra requires including ldra >, ldra suppressive activity results indicate present study novel function molecular basis membrane localization known role key factor homolog ldrd genetic screen findings reveal cytoplasmic domains |