DEPs based on subcellular localization.
<div><p>Objective</p><p>Radiation-induced brain injury (RIBI) is a significant complication following radiotherapy for brain tumors, leading to neurocognitive deficits and other neurological impairments. This study aims to identify potential biomarkers and therapeutic targets...
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2025
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| _version_ | 1849927628511772672 |
|---|---|
| author | Jing Liu (38537) |
| author2 | Junshuang Wang (22683435) Shuang Lv (660117) Hengjiao Wang (21539904) Defu Yang (735577) Ying Zhang (40767) Ying Li (38224) Huiling Qu (6104225) Ying Xu (9172) Ying Yan (47692) |
| author2_role | author author author author author author author author author |
| author_facet | Jing Liu (38537) Junshuang Wang (22683435) Shuang Lv (660117) Hengjiao Wang (21539904) Defu Yang (735577) Ying Zhang (40767) Ying Li (38224) Huiling Qu (6104225) Ying Xu (9172) Ying Yan (47692) |
| author_role | author |
| dc.creator.none.fl_str_mv | Jing Liu (38537) Junshuang Wang (22683435) Shuang Lv (660117) Hengjiao Wang (21539904) Defu Yang (735577) Ying Zhang (40767) Ying Li (38224) Huiling Qu (6104225) Ying Xu (9172) Ying Yan (47692) |
| dc.date.none.fl_str_mv | 2025-11-25T18:29:09Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0337608.s003 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/dataset/DEPs_based_on_subcellular_localization_/30713856 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biophysics Biochemistry Medicine Cell Biology Genetics Molecular Biology Neuroscience Pharmacology Evolutionary Biology Cancer Mental Health Infectious Diseases Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified tandem mass tagging resolution mass spectrometry 30 gy ). induced brain injury ribi &# 8217 ppi network analysis key hub protein kegg analysis highlighted identifying key proteins xlink "> radiation identify potential biomarkers xlink "> potential biomarkers potential role brain tumors brain tissues brain tissue brain irradiation tight junctions therapeutic targets targeted therapies study aims ribi pathophysiology rat model protein interaction ppar signaling pathways related pathways involved pathway analyses neurological impairments neurocognitive deficits molecular pathology molecular mechanisms mitigate radiation metabolic processes mainly involved lipid transport kyoto encyclopedia irradiated rats increased expression gene ontology findings suggest could serve clinical intervention cell adhesion biological processes associated pathways |
| dc.title.none.fl_str_mv | DEPs based on subcellular localization. |
| dc.type.none.fl_str_mv | Dataset info:eu-repo/semantics/publishedVersion dataset |
| description | <div><p>Objective</p><p>Radiation-induced brain injury (RIBI) is a significant complication following radiotherapy for brain tumors, leading to neurocognitive deficits and other neurological impairments. This study aims to identify potential biomarkers and therapeutic targets for RIBI by utilizing advanced proteomic techniques to explore the molecular mechanisms underlying RIBI.</p><p>Methods</p><p>A rat model of RIBI was established and subjected to whole-brain irradiation (30 Gy). Tandem mass tagging (TMT)-based quantitative proteomics, combined with high-resolution mass spectrometry, was used to identify differentially expressed proteins (DEPs) in the brain tissues of irradiated rats. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted to identify the biological processes and pathways involved. Protein-protein interaction (PPI) networks were constructed to identify key hub proteins.</p><p>Results</p><p>A total of 35 DEPs were identified, including PHLDA3, APOE and CPE. GO enrichment analysis revealed that the DEPs were mainly involved in lipid transport, cell adhesion, and metabolic processes. KEGG analysis highlighted the enrichment of pathways related to metabolism, tight junctions, and PPAR signaling. APOE was identified as a key hub protein through PPI network analysis, indicating its potential role in RIBI pathophysiology. Immunohistochemistry further validated the increased expression of PHLDA3, APOE, and CPE in the brain tissue of irradiated rats.</p><p>Conclusion</p><p>This study provides valuable insights into the molecular mechanisms of RIBI by identifying key proteins and their associated pathways. The findings suggest that these proteins, particularly APOE and PHLDA3, could serve as potential biomarkers and therapeutic targets for clinical intervention in RIBI. These results not only enhance our understanding of RIBI’s molecular pathology but also open new avenues for the development of targeted therapies to mitigate radiation-induced neurotoxicity.</p></div> |
| eu_rights_str_mv | openAccess |
| id | Manara_f7cfd3bcc5736df10056fbab659a1f9f |
| identifier_str_mv | 10.1371/journal.pone.0337608.s003 |
| network_acronym_str | Manara |
| network_name_str | ManaraRepo |
| oai_identifier_str | oai:figshare.com:article/30713856 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | DEPs based on subcellular localization.Jing Liu (38537)Junshuang Wang (22683435)Shuang Lv (660117)Hengjiao Wang (21539904)Defu Yang (735577)Ying Zhang (40767)Ying Li (38224)Huiling Qu (6104225)Ying Xu (9172)Ying Yan (47692)BiophysicsBiochemistryMedicineCell BiologyGeneticsMolecular BiologyNeurosciencePharmacologyEvolutionary BiologyCancerMental HealthInfectious DiseasesBiological Sciences not elsewhere classifiedChemical Sciences not elsewhere classifiedtandem mass taggingresolution mass spectrometry30 gy ).induced brain injuryribi &# 8217ppi network analysiskey hub proteinkegg analysis highlightedidentifying key proteinsxlink "> radiationidentify potential biomarkersxlink ">potential biomarkerspotential rolebrain tumorsbrain tissuesbrain tissuebrain irradiationtight junctionstherapeutic targetstargeted therapiesstudy aimsribi pathophysiologyrat modelprotein interactionppar signalingpathways relatedpathways involvedpathway analysesneurological impairmentsneurocognitive deficitsmolecular pathologymolecular mechanismsmitigate radiationmetabolic processesmainly involvedlipid transportkyoto encyclopediairradiated ratsincreased expressiongene ontologyfindings suggestcould serveclinical interventioncell adhesionbiological processesassociated pathways<div><p>Objective</p><p>Radiation-induced brain injury (RIBI) is a significant complication following radiotherapy for brain tumors, leading to neurocognitive deficits and other neurological impairments. This study aims to identify potential biomarkers and therapeutic targets for RIBI by utilizing advanced proteomic techniques to explore the molecular mechanisms underlying RIBI.</p><p>Methods</p><p>A rat model of RIBI was established and subjected to whole-brain irradiation (30 Gy). Tandem mass tagging (TMT)-based quantitative proteomics, combined with high-resolution mass spectrometry, was used to identify differentially expressed proteins (DEPs) in the brain tissues of irradiated rats. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted to identify the biological processes and pathways involved. Protein-protein interaction (PPI) networks were constructed to identify key hub proteins.</p><p>Results</p><p>A total of 35 DEPs were identified, including PHLDA3, APOE and CPE. GO enrichment analysis revealed that the DEPs were mainly involved in lipid transport, cell adhesion, and metabolic processes. KEGG analysis highlighted the enrichment of pathways related to metabolism, tight junctions, and PPAR signaling. APOE was identified as a key hub protein through PPI network analysis, indicating its potential role in RIBI pathophysiology. Immunohistochemistry further validated the increased expression of PHLDA3, APOE, and CPE in the brain tissue of irradiated rats.</p><p>Conclusion</p><p>This study provides valuable insights into the molecular mechanisms of RIBI by identifying key proteins and their associated pathways. The findings suggest that these proteins, particularly APOE and PHLDA3, could serve as potential biomarkers and therapeutic targets for clinical intervention in RIBI. These results not only enhance our understanding of RIBI’s molecular pathology but also open new avenues for the development of targeted therapies to mitigate radiation-induced neurotoxicity.</p></div>2025-11-25T18:29:09ZDatasetinfo:eu-repo/semantics/publishedVersiondataset10.1371/journal.pone.0337608.s003https://figshare.com/articles/dataset/DEPs_based_on_subcellular_localization_/30713856CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/307138562025-11-25T18:29:09Z |
| spellingShingle | DEPs based on subcellular localization. Jing Liu (38537) Biophysics Biochemistry Medicine Cell Biology Genetics Molecular Biology Neuroscience Pharmacology Evolutionary Biology Cancer Mental Health Infectious Diseases Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified tandem mass tagging resolution mass spectrometry 30 gy ). induced brain injury ribi &# 8217 ppi network analysis key hub protein kegg analysis highlighted identifying key proteins xlink "> radiation identify potential biomarkers xlink "> potential biomarkers potential role brain tumors brain tissues brain tissue brain irradiation tight junctions therapeutic targets targeted therapies study aims ribi pathophysiology rat model protein interaction ppar signaling pathways related pathways involved pathway analyses neurological impairments neurocognitive deficits molecular pathology molecular mechanisms mitigate radiation metabolic processes mainly involved lipid transport kyoto encyclopedia irradiated rats increased expression gene ontology findings suggest could serve clinical intervention cell adhesion biological processes associated pathways |
| status_str | publishedVersion |
| title | DEPs based on subcellular localization. |
| title_full | DEPs based on subcellular localization. |
| title_fullStr | DEPs based on subcellular localization. |
| title_full_unstemmed | DEPs based on subcellular localization. |
| title_short | DEPs based on subcellular localization. |
| title_sort | DEPs based on subcellular localization. |
| topic | Biophysics Biochemistry Medicine Cell Biology Genetics Molecular Biology Neuroscience Pharmacology Evolutionary Biology Cancer Mental Health Infectious Diseases Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified tandem mass tagging resolution mass spectrometry 30 gy ). induced brain injury ribi &# 8217 ppi network analysis key hub protein kegg analysis highlighted identifying key proteins xlink "> radiation identify potential biomarkers xlink "> potential biomarkers potential role brain tumors brain tissues brain tissue brain irradiation tight junctions therapeutic targets targeted therapies study aims ribi pathophysiology rat model protein interaction ppar signaling pathways related pathways involved pathway analyses neurological impairments neurocognitive deficits molecular pathology molecular mechanisms mitigate radiation metabolic processes mainly involved lipid transport kyoto encyclopedia irradiated rats increased expression gene ontology findings suggest could serve clinical intervention cell adhesion biological processes associated pathways |